{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T03:37:26Z","timestamp":1760240246947},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>Paracoccidioidomycosis is a systemic mycosis that is endemic to certain countries in Latin <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>merica. This study aimed to describe the histological features of liver involvement in patients with paracoccidioidomycosis aged &lt;16\u00a0years of age who were treated between 1980 and 2010, with a diagnosis that was confirmed by detection of the fungus by pathological examination.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Liver tissue was obtained from one necropsy and 12 biopsies. Throughout 2007, biopsies were taken from patients with persistent jaundice or portal hypertension, after which biopsies became indicated due to elevated aminotransferase and low albumin levels. Using haematoxylin and eosin (<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>&amp;<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>), Masson's trichrome and immunohistochemical (<jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>7 and <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>19) staining, we noted degenerative alterations in bile duct cells and inflammatory injury to the bile ducts in 10 biopsies. Using immunohistochemistry for <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>7 and <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>19, we observed ductal proliferation in all 12 samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Bile duct injuries by inflammatory cells might explain the predominant increase in canalicular enzymes; immunohistochemistry is more sensitive in demonstrating ductular reactions and might show changes that are not apparent on <jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>&amp;<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content> staining.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12244","type":"journal-article","created":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T16:46:58Z","timestamp":1375375618000},"page":"256-262","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Hepatic involvement in paediatric patients with paracoccidioidomycosis: a histological study"],"prefix":"10.1111","volume":"64","author":[{"given":"Giselle","family":"de Melo Braga","sequence":"first","affiliation":[{"name":"Center for Investigation in Pediatrics UNICAMP School of Medicine Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriel","family":"Hessel","sequence":"additional","affiliation":[{"name":"Department of Pediatrics UNICAMP School of Medicine Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo Mendes","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Pediatrics UNICAMP School of Medicine Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cec\u00edlia Am\u00e9lia Fazzio","family":"Escanhoela","sequence":"additional","affiliation":[{"name":"Departments of Pathology UNICAMP School of Medicine Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.6.2.89"},{"key":"e_1_2_6_3_1","first-page":"85","volume-title":"Paracoccidioidomicose (Blastomicose sul\u2010americana). 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