{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:59:22Z","timestamp":1761541162289},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,11,23]],"date-time":"2013-11-23T00:00:00Z","timestamp":1385164800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2014,3]]},"abstract":"Aims<\/jats:title>Gastric gland mucin contains O<\/jats:italic>\u2010glycans exhibiting terminal \u03b11,4\u2010linked N<\/jats:italic>\u2010acetylglucosamine residues (\u03b1GlcNA<\/jats:styled-content>c). Recently we demonstrated that mice deficient in \u03b1GlcNA<\/jats:styled-content>c in gastric gland mucin develop gastric adenocarcinoma spontaneously, indicating that \u03b1GlcNA<\/jats:styled-content>c is a tumour suppressor for gastric cancer. However, the role of \u03b1GlcNA<\/jats:styled-content>c in Barrett's oesophagus (BO<\/jats:styled-content>) remains unknown. In this study, we investigated whether reduced \u03b1GlcNA<\/jats:styled-content>c expression in BO<\/jats:styled-content> is associated with development of Barrett's adenocarcinoma (BAC<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods and results<\/jats:title>Thirty\u2010five BO<\/jats:styled-content> lesions adjacent to BAC<\/jats:styled-content> were examined by immunohistochemistry for \u03b1GlcNA<\/jats:styled-content>c, MUC<\/jats:styled-content>6 and CDX<\/jats:styled-content>2. As controls, 35 BO<\/jats:styled-content> lesions without BAC<\/jats:styled-content> obtained from patients with oesophageal squamous cell carcinoma were also analysed. Expression of \u03b1GlcNA<\/jats:styled-content>c relative to its scaffold MUC<\/jats:styled-content>6 in BO<\/jats:styled-content> adjacent to BAC<\/jats:styled-content> was reduced significantly compared to control BO<\/jats:styled-content>. Decreased \u03b1GlcNA<\/jats:styled-content>c expression in BO<\/jats:styled-content> adjacent to BAC<\/jats:styled-content> was particularly significant in patients with smaller tumour size (<20\u00a0mm) and minimal invasion of tumour cells to the superficial muscularis mucosae. There was also a significant inverse correlation between \u03b1GlcNA<\/jats:styled-content>c and CDX<\/jats:styled-content>2 expression in BO<\/jats:styled-content> adjacent to BAC<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Decreased expression of \u03b1GlcNA<\/jats:styled-content>c compared with MUC<\/jats:styled-content>6 in BO<\/jats:styled-content> is a possible hallmark in predicting BAC<\/jats:styled-content> development.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12296","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T01:40:58Z","timestamp":1381542058000},"page":"536-546","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Reduced expression of \u03b1G<\/scp>lcNA<\/scp>c in B<\/scp>arrett's oesophagus adjacent to B<\/scp>arrett's adenocarcinoma \u2013 a possible biomarker to predict the malignant potential of B<\/scp>arrett's oesophagus"],"prefix":"10.1111","volume":"64","author":[{"given":"Yugo","family":"Iwaya","sequence":"first","affiliation":[{"name":"Department of Molecular Pathology Shinshu University Graduate School of Medicine Matsumoto Japan"},{"name":"Department of Gastroenterology Shinshu University School of Medicine Matsumoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osamu","family":"Hasebe","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Nagano Municipal Hospital Nagano Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naohiko","family":"Koide","sequence":"additional","affiliation":[{"name":"Department of Surgery Shinshu University School of Medicine Matsumoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kei","family":"Kitahara","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Nagano Prefectural Kiso Hospital Kiso 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