{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T02:12:21Z","timestamp":1772849541230,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,12,31]],"date-time":"2013-12-31T00:00:00Z","timestamp":1388448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The purpose of this study was to examine the correlation between nuclear expression of cyclin\u2010dependent kinase 4 (<jats:styled-content style=\"fixed-case\">CDK<\/jats:styled-content>4) and clinicopathological data in nasopharyngeal carcinoma (<jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>), including patient survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Using real\u2010time PCR and immunohistochemistry, the expression of <jats:italic>CDK4<\/jats:italic> was examined in NPC and nasopharyngeal (NP) tissues. We observed that <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression of <jats:italic>CDK4<\/jats:italic> was elevated significantly in NPC tissues compared to NP tissues. Further, we found that CDK4 protein was expressed in both the nucleus and cytoplasm. Nuclear expression of CDK4 was correlated positively with clinical stage (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.048), but not associated with other clinical features. Patients with tumours showing nuclear expression of CDK4 had poorer overall survival rates than those without nuclear tumour expression of CDK4. Nuclear expression of CDK4 was associated inversely with survival time for NPC patients in stages T1\u20132, stages N2\u20133 and clinical stages III\u2013IV, and after treatment with radiotherapy or chemotherapy. Nuclear expression of CDK4 was an independent and unfavourable prognostic factor for patients with NPC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggest that nuclear expression of <jats:styled-content style=\"fixed-case\">CDK<\/jats:styled-content>4 is a potential marker for the progression and poor prognosis of <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12319","type":"journal-article","created":{"date-parts":[[2013,10,29]],"date-time":"2013-10-29T16:19:17Z","timestamp":1383063557000},"page":"722-730","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Nuclear expression of <scp>CDK<\/scp>4 correlates with disease progression and poor prognosis in human nasopharyngeal carcinoma"],"prefix":"10.1111","volume":"64","author":[{"given":"Qingping","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Pathology Third Affiliated Hospital of Guangzhou Medical University Guangzhou China"},{"name":"Department of Pathology Guangzhou Medical University Guangzhou China"},{"name":"Cancer Research Institute Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunping","family":"Mai","sequence":"additional","affiliation":[{"name":"Cancer Research Institute Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiling","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Pharmacy Guangdong Medical College Dongguan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiangyun","family":"Wu","sequence":"additional","affiliation":[{"name":"Cancer Research Institute Southern Medical University Guangzhou 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Southern Medical University Guangzhou China"},{"name":"Otorhinolaryngology of Zhujiang Hospital Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weiyi","family":"Fang","sequence":"additional","affiliation":[{"name":"Cancer Research Institute Southern Medical University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Pathology Third Affiliated Hospital of Guangzhou Medical University Guangzhou China"},{"name":"Department of Pathology Guangzhou Medical University Guangzhou China"},{"name":"Cancer Research Institute Southern Medical University Guangzhou 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