{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T14:43:12Z","timestamp":1785681792982,"version":"3.56.0"},"reference-count":18,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,2,25]],"date-time":"2014-02-25T00:00:00Z","timestamp":1393286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>We performed a head\u2010to\u2010head comparison of an antibody against uroplakin <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>3) and a new uroplakin <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>2) antibody that remains untested in diagnostically challenging settings.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We immunostained high\u2010grade bladder neck carcinomas (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>35), high\u2010grade upper tract urothelial carcinomas (<jats:styled-content style=\"fixed-case\">UC<\/jats:styled-content>) and renal carcinomas (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>85), metastases of <jats:styled-content style=\"fixed-case\">UC<\/jats:styled-content> (<jats:italic>n\u00a0=\u00a0<\/jats:italic>30) and a multicancer tissue microarray (<jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>88) for <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>3 and <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>2, and scored staining intensity and proportion. <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>3 showed membranous plaque\u2010like expression, while <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>2 staining showed both membranous and cytoplasmic positivity. Significantly greater intensity (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.003) and proportion (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.03) of staining was noted for <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>2 among bladder neck lesions, with <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>2 staining showing greater sensitivity (63% versus 19%) and similar specificity (95% versus 100%) for <jats:styled-content style=\"fixed-case\">UC<\/jats:styled-content> over prostate carcinoma (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.02). Among upper tract lesions, <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>2 staining showed greater intensity and proportion than <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>3 (both <jats:italic>P\u00a0<\/jats:italic>&lt;\u00a00.001), including improved sensitivity (68% versus 23%) and equal specificity (both 100%) for <jats:styled-content style=\"fixed-case\">UC<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.006). Among <jats:styled-content style=\"fixed-case\">UC<\/jats:styled-content> metastases, <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>2 staining showed greater intensity and proportion (both <jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001) with higher sensitivity (73% versus 37%, respectively, <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.001). Of 88 additional cases tested, no non\u2010urothelial cases stained for either <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>2 antibody outperforms the <jats:styled-content style=\"fixed-case\">UP<\/jats:styled-content>3 antibody, including in diagnostically challenging settings, and is a useful addition to the armamentarium of biomarkers for <jats:styled-content style=\"fixed-case\">UC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12360","type":"journal-article","created":{"date-parts":[[2014,1,2]],"date-time":"2014-01-02T08:47:04Z","timestamp":1388652424000},"page":"132-138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["Uroplakin <scp>II<\/scp> outperforms uroplakin <scp>III<\/scp> in diagnostically challenging settings"],"prefix":"10.1111","volume":"65","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0982-4607","authenticated-orcid":false,"given":"Steven C","family":"Smith","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Cedars\u2010Sinai Medical Center Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sambit K","family":"Mohanty","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Cedars\u2010Sinai Medical Center Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lakshmi P","family":"Kunju","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Michigan School of Medicine Ann Arbor MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Cedars\u2010Sinai Medical Center Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fai","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Cedars\u2010Sinai Medical Center Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason C","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Cedars\u2010Sinai Medical Center Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gladel P","family":"Paner","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Chicago Chicago IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna E","family":"Hansel","sequence":"additional","affiliation":[{"name":"Department of Pathology University of California, San Diego La Jolla CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel J","family":"Luthringer","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Cedars\u2010Sinai Medical Center Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariza N","family":"de Peralta\u2010Ventrurina","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Cedars\u2010Sinai Medical Center Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahul B","family":"Amin","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Cedars\u2010Sinai Medical Center Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,25]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/ki.2009.73"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.pas.0000213438.01278.5f"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3182a0218f"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2012.08.004"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/PYQC-17CB-063T-Q07J"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/hupa.2002.124787"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/hupa.2002.129416"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200301000-00001"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2011-0575-OA"},{"issue":"2","key":"e_1_2_5_11_1","first-page":"1A","article-title":"A newly developed anti\u2010uroplakin II monoclonal antibody with increased sensitivity in urothelial carcinoma of the bladder","volume":"26","author":"Hoang L","year":"2013","journal-title":"Mod. Pathol."},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.04093.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3182635954"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2013.07.006"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.76"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31829e2525"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318260cde7"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2012.05.018"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1309\/V1RY91NKX5ARW2Q5"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhis.12360","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.12360","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T01:14:02Z","timestamp":1696295642000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.12360"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,25]]},"references-count":18,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,7]]}},"alternative-id":["10.1111\/his.12360"],"URL":"https:\/\/doi.org\/10.1111\/his.12360","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,25]]},"assertion":[{"value":"2013-11-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-02-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}