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In this study, we aimed to characterize more clearly <jats:styled-content style=\"fixed-case\">VEGFA<\/jats:styled-content> gene and protein expression in <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>First, we compared <jats:italic>VEGFA <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels in MF and in normal T lymphocyte samples; significantly higher <jats:italic>VEGFA<\/jats:italic> levels were found in MF. We then studied <jats:italic>VEGFA<\/jats:italic> expression in different normal T cell subsets, focusing on CD4<jats:sup>+<\/jats:sup>, CD8<jats:sup>+<\/jats:sup>, resting and activated T lymphocytes. We applied the gene signatures of the normal T cell subsets to MF samples and found that activated T lymphocytes represented the closest normal counterpart of the tumour. However, <jats:italic>VEGFA <\/jats:italic><jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> levels were significantly higher in MF than in activated normal T cells, suggesting that <jats:italic>VEGFA<\/jats:italic> overexpression in MF represents an attribute acquired during neoplastic transformation: no significant <jats:italic>VEGFA<\/jats:italic> expression differences were recorded between early and advanced stages. Gene expression profile results were supported by immunohistochemistry in routine sections from 27 MF cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>For the first time, we demonstrate <jats:styled-content style=\"fixed-case\">VEGFA<\/jats:styled-content> expression in <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content> cells, suggesting that the <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> pathway may be implicated in <jats:styled-content style=\"fixed-case\">MF<\/jats:styled-content> pathogenesis and can represent a novel therapeutic target.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12445","type":"journal-article","created":{"date-parts":[[2014,4,26]],"date-time":"2014-04-26T06:48:40Z","timestamp":1398494920000},"page":"173-181","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Vascular endothelial growth factor A (<scp>VEGFA<\/scp>) expression in mycosis fungoides"],"prefix":"10.1111","volume":"66","author":[{"given":"Alessandro","family":"Pileri","sequence":"first","affiliation":[{"name":"Dermatology Unit Department Of Experimental Diagnostic and Specialty Medicine University of Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Agostinelli","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department Of Experimental Diagnostic and Specialty Medicine University of Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Righi","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department Of Experimental Diagnostic and Specialty Medicine University of Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"Fuligni","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department Of Experimental Diagnostic and Specialty Medicine University of Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Bacci","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department Of Experimental Diagnostic and Specialty Medicine University of Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Sabattini","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department Of Experimental Diagnostic and Specialty Medicine University of Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annalisa","family":"Patrizi","sequence":"additional","affiliation":[{"name":"Dermatology Unit Department Of Experimental Diagnostic and Specialty Medicine University of Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano A","family":"Pileri","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department Of Experimental Diagnostic and Specialty Medicine University of Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pier Paolo","family":"Piccaluga","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department Of Experimental Diagnostic and Specialty Medicine University of Bologna Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,11,10]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"296","volume-title":"WHO classification of tumours of haematopoietic and lymphoid tissues","author":"Swerdlow S","year":"2008"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2007.08.004"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-03-055749"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2010.32.0630"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-12-061507"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652410791316968"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.22783"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00119076"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1992.269"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(96)00396-6"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80108-7"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1197-1222"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199101033240101"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910550507"},{"key":"e_1_2_7_16_1","first-page":"401","article-title":"Tumor angiogenesis correlates with metastasis in invasive prostate carcinoma","volume":"143","author":"Weidner N","year":"1993","journal-title":"Am. 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