{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:09:46Z","timestamp":1790644186449,"version":"4.1.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,1,30]],"date-time":"2015-01-30T00:00:00Z","timestamp":1422576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The diagnosis of Hirschsprung's disease is currently based on the identification of aganglionosis and the presence of an increase in acetylcholinesterase\u2010positive hypertrophic nerve fibres in the large bowel submucosa. However, acetylcholinesterase staining is laborious and requires a skilled technician. The aim of this study was to identify a method for diagnosing Hirschsprung's disease reliably using an immunohistochemical panel of recently proposed markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Sixty\u2010nine specimens from 37 patients were evaluated. <jats:styled-content style=\"fixed-case\">MAP<\/jats:styled-content>2 and calretinin antibodies were shown to stain ganglia reliably in the submucosal and myenteric plexuses of normal tissue. By contrast, reduced staining of ganglia was observed in patients with Hirschsprung's disease. Staining for <jats:styled-content style=\"fixed-case\">GLUT<\/jats:styled-content>1 and S100 was used to evaluate the number and thickness of nerve fibres. Gain of <jats:styled-content style=\"fixed-case\">GLUT<\/jats:styled-content>1 and S100 expression was in contrast to the loss of calretinin and <jats:styled-content style=\"fixed-case\">MAP<\/jats:styled-content>2. Hypertrophic submucosal nerve fibres in Hirschsprung's disease develop a perineurium with a ring\u2010like <jats:styled-content style=\"fixed-case\">GLUT<\/jats:styled-content>1 staining pattern similar in size and intensity to that observed in deeper subserosal tissue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The diagnosis of Hirschsprung's disease using immunohistochemical panels could be as accurate as with conventional frozen section techniques. In particular, the use of a combination of markers for ganglia and hypertrophic nerve fibres highlighting a prominent perineurium in Hirschsprung's disease could be an alternative method.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12527","type":"journal-article","created":{"date-parts":[[2014,8,14]],"date-time":"2014-08-14T07:19:31Z","timestamp":1408000771000},"page":"824-835","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Immunohistochemical panel for the diagnosis of Hirschsprung's disease using antibodies to <scp>MAP<\/scp>2, calretinin, <scp>GLUT<\/scp>1 and S100"],"prefix":"10.1111","volume":"66","author":[{"given":"Leonhard","family":"Bachmann","sequence":"first","affiliation":[{"name":"Institute of Pathology Friedrich\u2010Alexander\u2010Universit\u00e4t Erlangen\u2010N\u00fcrnberg Erlangen Germany"},{"name":"Comprehensive Cancer Centre Erlangen\u00a0\u2013\u00a0Metropolitan Region Nuremberg Erlangen 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