{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T14:28:37Z","timestamp":1781015317999,"version":"3.54.1"},"reference-count":24,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,11,10]],"date-time":"2014-11-10T00:00:00Z","timestamp":1415577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>To compare Carnoy's solution (<jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content>) and 10% neutral buffered formalin solution (<jats:styled-content style=\"fixed-case\">NBF<\/jats:styled-content>) as tissue fixatives in colorectal cancer specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Surgical specimens from patients with colorectal cancer were analysed. Three groups were studied, as follows: group 1 consisted of 16 paired samples fixed in <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">NBF<\/jats:styled-content>; and groups 2 and 3 consisted of 14 prospective and 80 retrospective samples, respectively, both randomized for fixation in <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content> or <jats:styled-content style=\"fixed-case\">NBF<\/jats:styled-content>. Groups 1 and 2 were analysed for amount, quality and integrity of <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. Morphological analysis, including some of the usual special stains and polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>), were also performed for group 1, and Sanger sequencing for group 2. Immunohistochemical (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>) reactions for mismatch repair proteins were studied in groups 1 and 3. Fixative performances were similar for morphology, special stains, and <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> reactions, as well as for the amount, quality and integrity of extracted <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>. <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> amplification was not possible in two cases from <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content> group 1. Sanger sequencing gave conclusive results for the <jats:styled-content style=\"fixed-case\">CS<\/jats:styled-content> samples tested.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Carnoy's solution and <jats:styled-content style=\"fixed-case\">NBF<\/jats:styled-content> are equivalent fixatives for colorectal cancer specimens and are adequate for routine utilization in surgical and molecular pathology.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12532","type":"journal-article","created":{"date-parts":[[2014,10,12]],"date-time":"2014-10-12T08:12:12Z","timestamp":1413101532000},"page":"388-397","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Carnoy's solution is an adequate tissue fixative for routine surgical pathology, preserving cell morphology and molecular integrity"],"prefix":"10.1111","volume":"66","author":[{"given":"Marina A","family":"Pereira","sequence":"first","affiliation":[{"name":"Cancer Institute(ICESP) University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre R","family":"Dias","sequence":"additional","affiliation":[{"name":"Cancer Institute(ICESP) University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila F","family":"Faraj","sequence":"additional","affiliation":[{"name":"Cancer Institute(ICESP) University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cinthya dos S","family":"Cirqueira","sequence":"additional","affiliation":[{"name":"Cancer Institute(ICESP) University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele T","family":"Tomitao","sequence":"additional","affiliation":[{"name":"Cancer Institute(ICESP) University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Carlos Nahas","sequence":"additional","affiliation":[{"name":"Cancer Institute(ICESP) University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Ulysses","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Cancer Institute(ICESP) University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evandro S","family":"de Mello","sequence":"additional","affiliation":[{"name":"Cancer Institute(ICESP) University of Sao Paulo School of Medicine Sao Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,10]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Histotechnology: a self\u2010instructional text","author":"Carson FL","year":"2009"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2008.03148.x"},{"key":"e_1_2_8_4_1","volume-title":"World Health Organization classification of tumours of the digestive system","author":"Bosman FT","year":"2010"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-0956-3"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0101-98802009000300001"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1245\/ASO.2003.02.001"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v19.i46.8515"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4174\/jkss.2013.85.1.30"},{"key":"e_1_2_8_10_1","first-page":"31","article-title":"Modified clearing technique to identify lymph node metastases in post\u2010irradiated surgical specimens from rectal adenocarcinoma","volume":"27","author":"Sanchez W","year":"1996","journal-title":"Arch. 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