{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T19:04:25Z","timestamp":1758395065757},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,1,15]],"date-time":"2015-01-15T00:00:00Z","timestamp":1421280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the Natural Science Fund of Fujian Province, China","award":["2013D003"],"award-info":[{"award-number":["2013D003"]}]},{"name":"the Science and Technology Project of Xiamen Science and Technology Bureau, Fujian Province, China","award":["3502Z20134019"],"award-info":[{"award-number":["3502Z20134019"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Alterations in the expression of several long non\u2010coding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) have been found in primary nasopharyngeal carcinoma (<jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>). However, the effect of lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression on primary <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> as well as the molecular mechanism of lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> remains vague. This study was to identify differentially expressed lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s involved in <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> on a genome\u2010wide scale and predict their potential functions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Using high\u2010throughput microarray with 30\u00a0586 lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> and 26\u00a0109 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> probes, 856 lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and 767 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s were expressed differentially between <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> and chronic nasopharyngitis tissues. Bioinformatic analysis (clustering analysis, gene ontology analysis and pathway analysis) was used for further research. Differentially expressed lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were subgrouped into three types and differentially expressed <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s were clustered into 28 pathways. The first coexpression network analysis revealed that 46 lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s interacting with three <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s involved the Janus kinase\u2013signal transducer and activator of transcription (<jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">STAT<\/jats:styled-content>) signalling pathway. Quantitative real\u2010time polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) verified 11 up\u2010 and down\u2010regulated lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and eight <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>. The second coexpression network analysis showed that 23 significantly aberrantly expressed <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content>s interacted with three validated lncRNAs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study could provide new insight into the molecular mechanisms of lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and their potential role in <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> for further study. These differentially expressed lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s may act as novel biomarkers and therapeutic targets for <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12616","type":"journal-article","created":{"date-parts":[[2014,11,18]],"date-time":"2014-11-18T17:37:17Z","timestamp":1416332237000},"page":"1022-1030","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Genome\u2010wide analysis of long non\u2010coding <scp>RNA<\/scp> in primary nasopharyngeal carcinoma by microarray"],"prefix":"10.1111","volume":"66","author":[{"given":"Qing\u2010Qing","family":"Yang","sequence":"first","affiliation":[{"name":"Union School of Clinical Medicine Fujian Medical University Fuzhou Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan\u2010Fei","family":"Deng","sequence":"additional","affiliation":[{"name":"Union School of Clinical Medicine Fujian Medical University Fuzhou Fujian China"},{"name":"Department of Otolaryngology Zhongshan Hospital Xiamen University Xiamen Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,1,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044579X02000858"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3892\/mco.2014.262"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0360-3016(03)00117-2"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12092"},{"key":"e_1_2_7_6_1","first-page":"692","article-title":"Expression of long noncoding RNA MALAT1 gene in human nasopharyngeal carcinoma cell lines and its biological significance","volume":"33","author":"Xie L","year":"2013","journal-title":"Nan Fang Yi Ke Da Xue Xue Bao"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/404567"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0105538"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-11-225"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2013.02.083"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11596-013-1078-y"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0085599"},{"key":"e_1_2_7_13_1","first-page":"1286","article-title":"Long non\u2010coding RNAs as novel biomarkers and therapeutic targets in head and neck cancers","volume":"7","author":"Yang QQ","year":"2014","journal-title":"Int. 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