{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,6]],"date-time":"2026-01-06T13:59:13Z","timestamp":1767707953828,"version":"3.37.3"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,2,12]],"date-time":"2015-02-12T00:00:00Z","timestamp":1423699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Sci-Tech Special Project of China","award":["No. 2012ZX10002-011-005"],"award-info":[{"award-number":["No. 2012ZX10002-011-005"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["No. 81401945"],"award-info":[{"award-number":["No. 81401945"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"the Youth Project of Fujian province health department","award":["NO. 2013-2-89"],"award-info":[{"award-number":["NO. 2013-2-89"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Pygopus\u20102 (Pygo2) is a critical element of the canonical Wnt\/\u03b2\u2010catenin transcriptional complex. The aim of the present study was to investigate the expression patterns and clinicopathological significance of Pygo2 in human primary hepatocellular carcinoma (<jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Real\u2010time polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) analysis of the m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> levels of Pygo2 in 50 paired <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> cancer\/adjacent non\u2010cancerous tissues showed that Pygo2 m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression was significantly higher in cancerous tissues (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.009). Immunohistochemical analysis showed that abnormal Pygo2 protein expression in <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> patients was associated with age (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.025), tumour size (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.005), intra\u2010 or extra\u2010hepatic metastasis (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.029), vascular invasion (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.026) and tumour differentiation (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.004). Patients with normal Pygo2 protein expression showed a longer survival time (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.031) and a higher 1\u2010year survival rate (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.032) than those with abnormal Pygo2 expression. Cox's proportional hazard regression model showed that abnormal Pygo2 protein expression was a risk factor associated with the prognosis of <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> patients (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.043).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>To the best of our knowledge, this is the first report investigating Pygo2 expression patterns and their clinicopathological significance in <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>. Our findings suggest that Pygo2 may be an important predictor of poor outcome in <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content> patients, and could serve as a novel biomarker for <jats:styled-content style=\"fixed-case\">HCC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12637","type":"journal-article","created":{"date-parts":[[2014,12,26]],"date-time":"2014-12-26T13:53:32Z","timestamp":1419602012000},"page":"176-184","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Abnormal nuclear expression of Pygopus\u20102 in human primary hepatocellular carcinoma correlates with a poor prognosis"],"prefix":"10.1111","volume":"67","author":[{"given":"Sheng","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of General Surgery Xiehe Hospital Fujian Medical University Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery ZhongShan hospital Xiamen University Fujian China"},{"name":"Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma (Xiamen University Affiliated Zhongshan Hospital) Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fei","family":"He","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery ZhongShan hospital Xiamen University Fujian China"},{"name":"Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma (Xiamen University Affiliated Zhongshan Hospital) Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Min","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of General Surgery Xiehe Hospital Fujian Medical University Fujian China"},{"name":"Department of Hepatobiliary Surgery ZhongShan hospital Xiamen University Fujian China"},{"name":"Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma (Xiamen University Affiliated Zhongshan Hospital) Fujian China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,2,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25516"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2006.09.009"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.17.4089"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20757"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/dvg.20299"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2008.03.011"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200810133"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20090134"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00465-10"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb786"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2004.04.007"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.001495"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-2433"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12015-013-9466-z"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2008.05.553"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-5-15"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1311395111"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2013.04.012"},{"key":"e_1_2_7_20_1","first-page":"357","article-title":"Requirement of Pygopus 2 in breast cancer","volume":"30","author":"Andrews PG","year":"2007","journal-title":"Int. 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