{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:27:55Z","timestamp":1792798075165,"version":"4.5.0"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,3,12]],"date-time":"2015-03-12T00:00:00Z","timestamp":1426118400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"S\u00c3\u00a3o Paulo Research Foundation - FAPESP","award":["2011\/23204-5 AND 2011\/23366-5"],"award-info":[{"award-number":["2011\/23204-5 AND 2011\/23366-5"]}],"id":[{"id":"https:\/\/ror.org\/02ddkpn78","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      Metastasizing pleomorphic adenoma (\n                      <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>\n                      ) is a rare tumour, and its mechanism of metastasis still is unknown. To date, there has been no study on\n                      <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>\n                      genomics. We analysed primary and secondary\n                      <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>\n                      s with array comparative genomic hybridization to identify somatic copy number alterations and affected genes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>\n                      Tumour\n                      <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\n                      samples from primary (parotid salivary gland) and secondary (scalp skin)\n                      <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>\n                      s were subjected to array comparative genomic hybridization investigation, and the data were analysed with NEXUS COPY NUMBER DISCOVERY. The primary\n                      <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>\n                      showed copy number losses affecting 3p22.2p14.3 and 19p13.3p123, and a complex pattern of four different deletions at chromosome 6. The 3p deletion encompassed several genes:\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">CTNNB<\/jats:styled-content>\n                        1\n                      <\/jats:italic>\n                      ,\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">SETD<\/jats:styled-content>\n                        2\n                      <\/jats:italic>\n                      ,\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">BAP<\/jats:styled-content>\n                        1\n                      <\/jats:italic>\n                      , and\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">PBRM<\/jats:styled-content>\n                        1\n                      <\/jats:italic>\n                      , among others. The secondary\n                      <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>\n                      showed a genomic profile similar to that of the primary\n                      <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>\n                      , with acquisition of additional copy number changes affecting 9p24.3p13.1 (loss), 19q11q13.43 (gain), and 22q11.1q13.33 (gain).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our findings indicated a clonal origin of the secondary\n                      <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>\n                      , as both tumours shared a common profile of genomic copy number alterations. Furthermore, we were able to detect in the primary tumour a specific pattern of copy number alterations that could explain the metastasizing characteristic, whereas the secondary\n                      <jats:styled-content style=\"fixed-case\">MPA<\/jats:styled-content>\n                      showed a more unbalanced genome.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.12655","type":"journal-article","created":{"date-parts":[[2015,1,20]],"date-time":"2015-01-20T07:40:36Z","timestamp":1421739636000},"page":"410-415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Genomic copy number alterations of primary and secondary metastasizing pleomorphic adenomas"],"prefix":"10.1111","volume":"67","author":[{"given":"Fernanda Viviane","family":"Mariano","sequence":"first","affiliation":[{"name":"Pathology Department Faculty of Medicine State University of Campinas (UNICAMP) Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rog\u00e9rio de Oliveira","family":"Gondak","sequence":"additional","affiliation":[{"name":"Pathology Department Faculty of Santa Catarina (UFSC) Florian\u00f3polis Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Santos","family":"Martins","sequence":"additional","affiliation":[{"name":"Head and Neck Surgery Department Faculty of Medicine UNICAMP Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Della","family":"Coletta","sequence":"additional","affiliation":[{"name":"Oral Pathology Department Piracicaba Dental School UNICAMP Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oslei","family":"Paes de Almeida","sequence":"additional","affiliation":[{"name":"Oral Pathology Department Piracicaba Dental School UNICAMP Piracicaba Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz Paulo","family":"Kowalski","sequence":"additional","affiliation":[{"name":"Head and Neck Surgery Department AC Camargo Cancer Center S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cristina Victorino","family":"Krepischi","sequence":"additional","affiliation":[{"name":"Department of Genetics and Evolutionary Biology Institute of Biosciences University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albina","family":"Altemani","sequence":"additional","affiliation":[{"name":"Pathology Department Faculty of Medicine State University of Campinas (UNICAMP) Campinas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/pin.12087"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(97)00301-4"},{"key":"e_1_2_7_4_1","first-page":"245\u20101","volume-title":"World Health Organization classification of tumours, pathology & genetics, head and neck tumours","author":"Gnepp DR","year":"2005"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2009.07.008"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/08959374000140011301"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2003.12.001"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800386"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20619"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-011-1063-4"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-012-0353-8"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199209000-00003"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(195311)6:6<1065::AID-CNCR2820060602>3.0.CO;2-0"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0022215100106528"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.moo.0000153450.87288.2a"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.132.7.788"},{"key":"e_1_2_7_17_1","first-page":"193","article-title":"Metastatic benign pleomorphic adenoma. 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