{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T02:54:34Z","timestamp":1781924074507,"version":"3.54.5"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,3,8]],"date-time":"2015-03-08T00:00:00Z","timestamp":1425772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate redox\u2010regulating enzymes that may have a special role in melanoma pathogenesis due to continuous exposure to microenvironment\u2010produced and ultraviolet radiation\u2010induced oxidative stress.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We assessed immunohistochemically the expression of antioxidant enzymes peroxiredoxins (Prxs) I\u2013<jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>, sulfiredoxin (Srx) and redox\u2010regulated proto\u2010oncogene <jats:styled-content style=\"fixed-case\">DJ<\/jats:styled-content>\u20101 in material consisting of 30 benign naevi, 14 lentigo malignas and 67 malignant melanomas. Evaluation of immunostaining was performed with special attention paid to protein expression in different tumour compartments. In particular, the expression patterns of nuclear Prx I and Prx <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> and cytoplasmic <jats:styled-content style=\"fixed-case\">DJ<\/jats:styled-content>\u20101 were decreased significantly in melanomas compared with dysplastic and benign naevi. In multivariate analysis, several prognostic factors were identified: Prx <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> expression in the cytoplasm of stromal fibroblasts was associated with shortened melanoma\u2010specific survival [hazard ratio (<jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>) 6.730; 95% confidence interval (<jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>) 1.579\u201328.689], while cytoplasmic Prx <jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content> expression in endothelial cells (<jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> 6.563; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content> 1.750\u201324.620) and Srx expression in the cytoplasm of keratinocytes (<jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> 6.988; 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content> 1.559\u201331.324) were associated with better prognosis independently of ulceration, thickness of melanoma or its diagnostic type.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Redox\u2010regulating enzymes have the potential to serve as novel prognostic factors and targeting them may offer new therapeutic options in malignant melanoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12659","type":"journal-article","created":{"date-parts":[[2015,1,28]],"date-time":"2015-01-28T05:21:08Z","timestamp":1422422468000},"page":"348-357","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Dysregulation of redox\u2010state\u2010regulating enzymes in melanocytic skin tumours and the surrounding microenvironment"],"prefix":"10.1111","volume":"67","author":[{"given":"Hanna\u2010Riikka","family":"Hintsala","sequence":"first","affiliation":[{"name":"Institute of Clinical Medicine Pathology and Forensic Medicine University of Eastern Finland Cancer Center of Eastern Finland Kuopio Finland"},{"name":"Department of Clinical Pathology Kuopio University Hospital Kuopio Finland"},{"name":"University of Oulu and Department of Pathology Oulu University Hospital Oulu Finland"},{"name":"Department of Oncology and Radiotherapy Oulu University Hospital and University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ylermi","family":"Soini","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine Pathology and Forensic Medicine University of Eastern Finland Cancer Center of Eastern Finland Kuopio Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsi\u2010Maria","family":"Haapasaari","sequence":"additional","affiliation":[{"name":"University of Oulu and Department of Pathology Oulu University Hospital Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peeter","family":"Karihtala","sequence":"additional","affiliation":[{"name":"Department of Oncology and Radiotherapy Oulu University Hospital and University of Oulu Oulu Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/145749690209100206"},{"key":"e_1_2_7_3_1","first-page":"50","volume-title":"Pathology and genetics of tumours of the skin. 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