{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T04:29:07Z","timestamp":1788236947216,"version":"build-2803163510"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,3,8]],"date-time":"2015-03-08T00:00:00Z","timestamp":1425772800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"Aims<\/jats:title>Metallothioneins (MT<\/jats:styled-content>s) are proteins associated with the carcinogenesis and prognosis of various tumours. Previous studies have shown their potential as biomarkers in oral squamous cell carcinoma (OSCC<\/jats:styled-content>). Aiming to understand more clearly the function of MT<\/jats:styled-content>s in OSCC<\/jats:styled-content> we evaluated, for the first time, the gene expression profile of MT<\/jats:styled-content>s in this neoplasm.<\/jats:p><\/jats:sec>Materials and results<\/jats:title>Tissue samples from 35 cases of tongue and\/or floor of mouth OSCC<\/jats:styled-content>, paired with their corresponding non\u2010neoplastic oral mucosa (NNOM<\/jats:styled-content>), were retrieved (2007\u201309). All tissues were analysed for the following genes using TaqMan\u00ae<\/jats:sup> reverse transcription\u2013quantitative polymerase chain reaction (RT<\/jats:styled-content>\u2013qPCR<\/jats:styled-content>) assays: MT<\/jats:styled-content>1A<\/jats:italic>, MT<\/jats:styled-content>1B<\/jats:italic>, MT<\/jats:styled-content>1E<\/jats:italic>, MT<\/jats:styled-content>1F<\/jats:italic>, MT<\/jats:styled-content>1G<\/jats:italic>, MT<\/jats:styled-content>1H<\/jats:italic>, MT<\/jats:styled-content>1X<\/jats:italic>, MT<\/jats:styled-content>2A<\/jats:italic>, MT<\/jats:styled-content>3<\/jats:italic> and MT<\/jats:styled-content>4<\/jats:italic>. The expression of MT<\/jats:styled-content>1B and MT<\/jats:styled-content>1H was seldom detected in both OSCC<\/jats:styled-content> and NNOM<\/jats:styled-content>. A significant loss of MT<\/jats:styled-content>1A<\/jats:italic>, MT<\/jats:styled-content>1X<\/jats:italic>, MT<\/jats:styled-content>3<\/jats:italic> and MT<\/jats:styled-content>4<\/jats:italic> expression and gain of MT<\/jats:styled-content>1F<\/jats:italic> expression was observed in OSCC<\/jats:styled-content>, compared to NNOM<\/jats:styled-content>. Cases with MT<\/jats:styled-content>1G<\/jats:italic> down\u2010regulation exhibited the worst prognoses. The up\u2010regulation of MT<\/jats:styled-content>1X<\/jats:italic> was restricted to non\u2010metastatic cases, whereas up\u2010regulation of MT<\/jats:styled-content>3<\/jats:italic> was related to cases with lymph node metastasis.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Metallothionein mRNA<\/jats:styled-content> expression is altered significantly in oral squamous cell carcinomas. The expression of MT<\/jats:styled-content>1G<\/jats:italic>, MT<\/jats:styled-content>1X<\/jats:italic> and MT<\/jats:styled-content>3<\/jats:italic> may aid in the prognostic discrimination of OSCC<\/jats:styled-content> cases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12660","type":"journal-article","created":{"date-parts":[[2015,1,30]],"date-time":"2015-01-30T07:17:38Z","timestamp":1422602258000},"page":"358-367","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Metallothionein gene expression is altered in oral cancer and may predict metastasis and patient outcomes"],"prefix":"10.1111","volume":"67","author":[{"given":"Marco T","family":"Braz\u00e3o\u2010Silva","sequence":"first","affiliation":[{"name":"PhD program in Estomatology and Basic and Applied Pathology School of Dentistry University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Fernandes S","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Pathology School of Dentistry University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana L\u00facia A","family":"Eisenberg","sequence":"additional","affiliation":[{"name":"Department of Pathology Brazilian National Cancer Institute\/INCA Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando L","family":"Dias","sequence":"additional","affiliation":[{"name":"Department of Head and Neck Surgery Brazilian National Cancer Institute\/INCA Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana M","family":"de Castro","sequence":"additional","affiliation":[{"name":"National Tumor Bank Brazilian National Cancer Institute\/INCA Rio de Janeiro Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F\u00e1bio D","family":"Nunes","sequence":"additional","affiliation":[{"name":"PhD program in Estomatology and Basic and Applied Pathology School of Dentistry University of S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Laboratory of Molecular Pathology School of Dentistry University of S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Department of Oral Pathology School of Dentistry University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo R","family":"Faria","sequence":"additional","affiliation":[{"name":"Department of Histology and Morphology Institute of Biomedical Sciences Federal University of Uberl\u00e2ndia Uberl\u00e2ndia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1809-0617","authenticated-orcid":false,"given":"S\u00e9rgio V","family":"Cardoso","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology School of Dentistry Federal University of Uberl\u00e2ndia Uberl\u00e2ndia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano M","family":"Loyola","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology School of Dentistry Federal University of Uberl\u00e2ndia Uberl\u00e2ndia Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzana C O M","family":"de Sousa","sequence":"additional","affiliation":[{"name":"PhD program in Estomatology and Basic and Applied Pathology School of Dentistry University of S\u00e3o Paulo S\u00e3o Paulo Brazil"},{"name":"Department of Oral Pathology School of Dentistry University of S\u00e3o Paulo S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2003.07.013"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2004.01922.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.proghi.2008.10.001"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1039\/9781847559531-00001"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms14036044"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.7.3483"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2003.07.014"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1080\/10409230091169168"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(84)90322-2"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00189a029"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i7.993"},{"key":"e_1_2_7_13_1","first-page":"168","volume-title":"World Health Organization classification of tumours. 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