{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T22:16:38Z","timestamp":1783721798255,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,3,31]],"date-time":"2015-03-31T00:00:00Z","timestamp":1427760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Bile duct adenomas (<jats:styled-content style=\"fixed-case\">BDA<\/jats:styled-content>) and bile duct hamartomas (<jats:styled-content style=\"fixed-case\">BDH<\/jats:styled-content>) are benign bile duct lesions considered neoplastic or secondary to ductal plate malformation, respectively. We have reported previously a high prevalence of <jats:italic><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content><\/jats:italic> V600E mutations detected by allele\u2010specific polymerase chain reaction assay in <jats:styled-content style=\"fixed-case\">BDA<\/jats:styled-content>, and suggested that <jats:styled-content style=\"fixed-case\">BDA<\/jats:styled-content> may be precursors to a subset of intrahepatic cholangiocarcinomas harbouring V600E mutations. The aim of the present study was to assess the existence of <jats:italic><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content><\/jats:italic> V600E mutations, using immunohistochemical methods, in additional <jats:styled-content style=\"fixed-case\">BDA<\/jats:styled-content> as well as in <jats:styled-content style=\"fixed-case\">BDH<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Fifteen <jats:styled-content style=\"fixed-case\">BDA<\/jats:styled-content> and 35 <jats:styled-content style=\"fixed-case\">BDH<\/jats:styled-content> were retrieved from the archives of the pathology departments of two French university hospitals. All cases were reviewed by two pathologists specialized in liver diseases. <jats:italic><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content><\/jats:italic> V600E mutational status was investigated by immunohistochemistry. Mutated <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> mutant protein was detected in 53% of the <jats:styled-content style=\"fixed-case\">BDA<\/jats:styled-content> and in none of the cases of <jats:styled-content style=\"fixed-case\">BDH<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings suggest that <jats:styled-content style=\"fixed-case\">BDA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">BDH<\/jats:styled-content> are different processes, and that <jats:styled-content style=\"fixed-case\">BDA<\/jats:styled-content> represent true benign neoplasms. They also support the hypothesis that mutated <jats:styled-content style=\"fixed-case\">BDA<\/jats:styled-content> might precede the development of the subset of intrahepatic cholangiocarcinomas harbouring <jats:italic><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content><\/jats:italic> V600E mutations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12674","type":"journal-article","created":{"date-parts":[[2015,2,19]],"date-time":"2015-02-19T13:58:49Z","timestamp":1424354329000},"page":"562-567","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["<scp>BRAF<\/scp> V600E mutational status in bile duct adenomas and hamartomas"],"prefix":"10.1111","volume":"67","author":[{"given":"Ana\u00efs","family":"Pujals","sequence":"first","affiliation":[{"name":"Department of Pathology Assistance Publique\u2010H\u00f4pitaux de Paris CHU Henri Mondor Cr\u00e9teil France"},{"name":"Facult\u00e9 de M\u00e9decine Universit\u00e9 Paris\u2010Est Cr\u00e9teil Cr\u00e9teil France"},{"name":"Inserm U955 Equipe 9 Institut Mondor de Recherche Biom\u00e9dicale Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulette","family":"Bioulac\u2010Sage","sequence":"additional","affiliation":[{"name":"Department of Pathology CHU de Bordeaux Pellegrin Hospital Bordeaux France"},{"name":"Inserm UMR\u20101053 Universit\u00e9 de Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Castain","sequence":"additional","affiliation":[{"name":"Department of Pathology CHU de Bordeaux Pellegrin Hospital Bordeaux France"},{"name":"Inserm UMR\u20101053 Universit\u00e9 de Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Charpy","sequence":"additional","affiliation":[{"name":"Department of Pathology Assistance Publique\u2010H\u00f4pitaux de Paris CHU Henri Mondor Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elie Serge","family":"Zafrani","sequence":"additional","affiliation":[{"name":"Department of Pathology Assistance Publique\u2010H\u00f4pitaux de Paris CHU Henri Mondor Cr\u00e9teil France"},{"name":"Facult\u00e9 de M\u00e9decine Universit\u00e9 Paris\u2010Est Cr\u00e9teil Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Calderaro","sequence":"additional","affiliation":[{"name":"Department of Pathology Assistance Publique\u2010H\u00f4pitaux de Paris CHU Henri Mondor Cr\u00e9teil France"},{"name":"Facult\u00e9 de M\u00e9decine Universit\u00e9 Paris\u2010Est Cr\u00e9teil Cr\u00e9teil France"},{"name":"Inserm U955 Equipe 18 Institut Mondor de Recherche Biom\u00e9dicale Cr\u00e9teil France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,3,31]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"233","article-title":"The hepatic von Meyenburg complex: prevalence and association with hepatic and renal cysts among 2843 autopsies [corrected]","volume":"9","author":"Redston MS","year":"1996","journal-title":"Mod. 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