{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:22:34Z","timestamp":1740126154490,"version":"3.37.3"},"reference-count":16,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,27]],"date-time":"2015-04-27T00:00:00Z","timestamp":1430092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001308","name":"Jean Shanks Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001308","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2015,10]]},"abstract":"Aims<\/jats:title>The goal of this study was to pilot a commercial four\u2010colour fluorescence in\u2010situ<\/jats:italic> hybridization (FISH<\/jats:styled-content>) probe set as a marker of dysplasia in surveillance biopsies.<\/jats:p><\/jats:sec>Methods and results<\/jats:title>FISH<\/jats:styled-content> probes to 9p12 (CDKN<\/jats:styled-content>2A<\/jats:italic>), 17q11.2\u201012 (HER<\/jats:styled-content>2<\/jats:italic>), 8q24.12\u201013 (CMYC<\/jats:styled-content><\/jats:italic>) and 20q13.2 (ZNF<\/jats:styled-content>217<\/jats:italic>) in 20 cases of Barrett's oesophagus. Dysplastic and non\u2010dysplastic mucosa were compared for each case. Two observers independently counted 50 cells in each region of interest (ROI<\/jats:styled-content>), and the mean score taken. Wilcoxon's signed\u2010rank test was used to determine the significance of differences between dysplastic and non\u2010dysplastic tissue. Predictive power was determined by logistic regression and receiver operator characteristic (ROC<\/jats:styled-content>) curves were plotted to examine sensitivity and specificity of each gene to detect dysplasia. Interobserver agreement was excellent. HER<\/jats:styled-content>2<\/jats:italic>, CMYC<\/jats:styled-content><\/jats:italic> and ZNF<\/jats:styled-content>217<\/jats:italic> showed significant (P<\/jats:italic>\u00a0<\u00a00.0005) increases in copy number in dysplastic mucosa; CDKN<\/jats:styled-content>2A<\/jats:italic> had an insignificant (P\u00a0<\/jats:italic>=\u00a00.852) decrease when compared to non\u2010dysplastic mucosa. While aneusomy was strongly predictive of dysplasia, eusomy did not rule it out.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Increased HER<\/jats:styled-content>2<\/jats:italic>, CMYC<\/jats:styled-content><\/jats:italic> and ZNF<\/jats:styled-content>217<\/jats:italic> copy number distinguished dysplastic from non\u2010dysplastic mucosa, but non\u2010detection of aneusomy did\u00a0not exclude dysplasia. Further studies are justified to determine whether FISH<\/jats:styled-content>\u2010positive dysplasia might justify earlier treatment by radio\u2010frequency ablation.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12679","type":"journal-article","created":{"date-parts":[[2015,3,3]],"date-time":"2015-03-03T14:34:52Z","timestamp":1425393292000},"page":"451-456","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Aneusomy detected by fluorescence in\u2010situ<\/i> hybridization has high positive predictive value for B<\/scp>arrett's dysplasia"],"prefix":"10.1111","volume":"67","author":[{"given":"Euan A G","family":"Allan","sequence":"first","affiliation":[{"name":"University of Glasgow Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roy","family":"Miller","sequence":"additional","affiliation":[{"name":"Greater Glasgow and Clyde NHS Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James J","family":"Going","sequence":"additional","affiliation":[{"name":"Greater Glasgow and Clyde NHS University of Glasgow Glasgow UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0046-8177(94)90057-4"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.1999.01275.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2004.041525"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajg.2010.2"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2013-305506"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1136\/pgmj.2006.054841"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2014.2511"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.5946\/ce.2014.47.1.40"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1572-0241.2000.03348.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.050118"},{"key":"e_1_2_6_12_1","first-page":"404","article-title":"3p21, 5q21, 9p21 and 17p13.1 allelic deletions are potential markers of individuals with a high risk of developing adenocarcinoma in Barrett's epithelium without dysplasia","volume":"50","author":"Sanz\u2010Ortega J","year":"2003","journal-title":"Hepatogastroenterology"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.07.006"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.breast.2011.07.009"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2005.083600"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0079079"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2014.2682"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhis.12679","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.12679","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T20:09:09Z","timestamp":1696363749000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.12679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,27]]},"references-count":16,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,10]]}},"alternative-id":["10.1111\/his.12679"],"URL":"https:\/\/doi.org\/10.1111\/his.12679","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"type":"print","value":"0309-0167"},{"type":"electronic","value":"1365-2559"}],"subject":[],"published":{"date-parts":[[2015,4,27]]},"assertion":[{"value":"2014-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-03-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-04-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}