{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T07:49:50Z","timestamp":1696405790972},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,6,26]],"date-time":"2015-06-26T00:00:00Z","timestamp":1435276800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Nederlandse Organisatie voor Wetenschappelijk Onderzoek","doi-asserted-by":"publisher","award":["016.111.075"],"award-info":[{"award-number":["016.111.075"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>For patients who have multiple lymphomas with discordant pathology, it is relevant to determine whether there is one disseminated lymphoma or two unrelated lymphomas. Patients with disseminated, clonally related lymphomas are usually treated with the most powerful drugs available, while patients with unrelated (primary) lymphomas receive mainly standard first\u2010line therapies.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We used next\u2010generation sequencing on the Ion Torrent Personal Genome Machine to characterize the immunoglobulin heavy gene V\u2013D\u2013J rearrangements in two diagnostic tissue samples, including formalin\u2010fixed and paraffin\u2010embedded tissue, of two patients with iatrogenic immunodeficiency\u2010associated Epstein\u2013Barr virus lymphoproliferative disorder, with ulcerative colitis as underlying disease. The immunoglobulin rearrangement sequences obtained by next\u2010generation sequencing revealed undoubtedly clonally related lesions in two tissue biopsies that were taken over time in the first patient, which is concordant with disseminated lymphoma. The other patient showed two clonally unrelated lesions, which is incompatible with clonal dissemination. This information was not inferred from evaluation of the heavy and light chain rearrangements by fragment analysis, which is currently the gold standard.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study demonstrates the diagnostic application of next\u2010generation sequencing of immunoglobulin rearrangement assessment in pathology for clinical decision\u2010making in patients with several simultaneous or subsequent lymphoproliferations.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12714","type":"journal-article","created":{"date-parts":[[2015,4,18]],"date-time":"2015-04-18T07:58:56Z","timestamp":1429343936000},"page":"843-858","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Immunoglobulin rearrangement analysis from multiple lesions in the same patient using next\u2010generation sequencing"],"prefix":"10.1111","volume":"67","author":[{"given":"Silke","family":"Appenzeller","sequence":"first","affiliation":[{"name":"Center for Molecular and Biomolecular Informatics Radboud Institute for Molecular Life Sciences Radboud University Medical Center Nijmegen The Netherlands"},{"name":"Core Unit Systems Medicine University of Wuerzburg Wuerzburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Gilissen","sequence":"additional","affiliation":[{"name":"Department of Genetics Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos","family":"Rijntjes","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bastiaan B J","family":"Tops","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud 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