{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T11:49:58Z","timestamp":1772797798004,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,7,27]],"date-time":"2015-07-27T00:00:00Z","timestamp":1437955200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>To assess whether in oligoastrocytomas <jats:styled-content style=\"fixed-case\">ATRX<\/jats:styled-content> deficiency, as a surrogate of the alternative lengthening of telomeres (<jats:styled-content style=\"fixed-case\">ALT<\/jats:styled-content>) pathway,\u00a0has a role in predicting the presence or absence of loss of heterozygosity (<jats:styled-content style=\"fixed-case\">LOH<\/jats:styled-content>) of 1p and 19q, the genetic signature of oligodendroglial differentiation and a favourable prognostic marker.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>A series of 54 oligoastrocytomas were investigated by immunohistochemistry as well as microsatellite analysis for <jats:styled-content style=\"fixed-case\">LOH<\/jats:styled-content> 1p19q. Genetic findings were correlated with morphological assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">ATRX<\/jats:styled-content> deficiency was mutually exclusive with <jats:styled-content style=\"fixed-case\">LOH<\/jats:styled-content>. Conversely, <jats:styled-content style=\"fixed-case\">ATRX<\/jats:styled-content>\u2010proficient tumours immunoreactive for R132H\u2010mutant isocitrate dehydrogenase 1 (<jats:styled-content style=\"fixed-case\">IDH<\/jats:styled-content>1) showed a high rate (85%) of <jats:styled-content style=\"fixed-case\">LOH<\/jats:styled-content>. A more oligodendroglioma\u2010like morphology was associated with a higher rate of <jats:styled-content style=\"fixed-case\">LOH<\/jats:styled-content> even in the morphologically ambiguous group of oligoastrocytomas. Our findings support the concept that oligoastrocytomas represent a morphological grey zone, rather than a group of truly \u2018mixed\u2019 or \u2018intermediate\u2019 tumours. More precise classification of diffuse gliomas may also improve grading of borderline cases. We propose an immunohistochemical algorithm for classification of morphologically ambiguous diffuse gliomas.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12743","type":"journal-article","created":{"date-parts":[[2015,5,27]],"date-time":"2015-05-27T14:51:50Z","timestamp":1432738310000},"page":"272-278","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Combined <scp>ATRX<\/scp>\/<scp>IDH<\/scp>1 immunohistochemistry predicts genotype of oligoastrocytomas"],"prefix":"10.1111","volume":"68","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9128-0364","authenticated-orcid":false,"given":"Ekkehard","family":"Hewer","sequence":"first","affiliation":[{"name":"Institute of Pathology University of Bern Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Istvan","family":"Vajtai","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of Bern Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias S","family":"Dettmer","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of Bern Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabina","family":"Berezowska","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of Bern Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik","family":"Vassella","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of Bern Bern Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,7,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2002.tb00440.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.837"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005072-199501000-00011"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-014-1326-7"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-014-1359-y"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.100680"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.080958"},{"key":"e_1_2_6_9_1","volume-title":"WHO classification of tumours of the central nervous system","author":"Louis D","year":"2007"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2012.00630.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1207313"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2011.06.018"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-11-104"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prp.2012.09.009"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-006-0051-2"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/neup.12097"},{"key":"e_1_2_6_17_1","unstructured":"LowryR.Vassarstats. 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