{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T23:09:36Z","timestamp":1772665776762,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T00:00:00Z","timestamp":1445558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Primary sclerosing cholangitis (<jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content>) is characterized histologically by portal inflammation, bile duct injury and regeneration and concentric periductal fibrosis. Although seen commonly in our experience, the significance of histological thickening of the bile duct basement membrane on periodic acid Schiff (<jats:styled-content style=\"fixed-case\">PAS<\/jats:styled-content>)\u2010positive, diastase\u2010resistant (<jats:styled-content style=\"fixed-case\">DPAS<\/jats:styled-content>) staining has never been analysed formally. In this paper we provide an evidence\u2010based assessment of basement membrane thickening (<jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content>) reproducibility and diagnostic accuracy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>A total of 128 archived medical liver core biopsies were retrieved and blinded for review by two independent histopathologists. <jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content> was assessed and designated as absent or present with a grade (G) of G1\u2013G3. The sensitivity of any <jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content> for <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content> was good at 77%, with moderate specificity at 61%. When only G3 <jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content> was considered positive, the specificity was high at 95% but the sensitivity was poor at 16%. The interobserver agreement (0.69) and consistency (0.72) were good.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Basement membrane thickening is a reproducible predictor for <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content> with good sensitivity and specificity. The presence of G2 and especially G3 <jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content> showed high specificity and could be regarded as highly predictive of <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content>. The presence of more than G1 <jats:styled-content style=\"fixed-case\">BMT<\/jats:styled-content> should be reported and the possibility of <jats:styled-content style=\"fixed-case\">PSC<\/jats:styled-content> should be raised in the differential diagnosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12857","type":"journal-article","created":{"date-parts":[[2015,9,3]],"date-time":"2015-09-03T11:00:08Z","timestamp":1441278008000},"page":"819-824","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Bile duct basement membrane thickening in primary sclerosing cholangitis"],"prefix":"10.1111","volume":"68","author":[{"given":"Richard","family":"Colling","sequence":"first","affiliation":[{"name":"Cellular Pathology John Radcliffe Hospital Oxford University Hospitals NHS Trust Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clare","family":"Verrill","sequence":"additional","affiliation":[{"name":"Cellular Pathology John Radcliffe Hospital Oxford University Hospitals NHS Trust Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eve","family":"Fryer","sequence":"additional","affiliation":[{"name":"Cellular Pathology John Radcliffe Hospital Oxford University Hospitals NHS Trust Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christiana","family":"Kartsonaki","sequence":"additional","affiliation":[{"name":"Department of Oncology University of Oxford Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lai M","family":"Wang","sequence":"additional","affiliation":[{"name":"Cellular Pathology John Radcliffe Hospital Oxford University Hospitals NHS Trust Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Chapman","sequence":"additional","affiliation":[{"name":"Medical Sciences Division University of Oxford Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naayil","family":"Rajabally","sequence":"additional","affiliation":[{"name":"Department of Hepatology Oxford University Hospitals NHS Trust Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth","family":"Fleming","sequence":"additional","affiliation":[{"name":"Medical Sciences Division University of Oxford Oxford UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2013.03.015"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2013.06.052"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000358150"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2013.02.016"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.5582\/irdr.2014.01018"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11894-013-0361-1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1586\/egh.10.33"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1586\/17474124.2014.852468"},{"key":"e_1_2_7_10_1","first-page":"43","article-title":"Surgical pathology of the syndrome of primary sclerosing cholangitis","volume":"13","author":"Ludwig J","year":"1989","journal-title":"Am. 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