{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T02:43:54Z","timestamp":1790822634309,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,2,23]],"date-time":"2016-02-23T00:00:00Z","timestamp":1456185600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2016,8]]},"abstract":"Aims<\/jats:title>We performed an immunohistochemical analysis of alpha\u2010thalassaemia\/mental retardation syndrome X\u2010linked (ATRX<\/jats:styled-content>) expression in adult diffuse gliomas, with reference to clinicopathological and genetic features, to determine the utility of this analysis in diagnostic practice.<\/jats:p><\/jats:sec>Methods and results<\/jats:title>A total of 193 adult diffuse gliomas underwent immunohistochemical analysis. In areas in which internal controls, neurones, glia and blood vessels were properly stained, the ATRX<\/jats:styled-content> immunoreactivity of tumour cells was either almost totally absent or completely retained in all cases. There was perfect concordance between the immunohistochemical results and ATRX<\/jats:styled-content><\/jats:italic> mutation status, which was known in 19 cases. ATRX<\/jats:styled-content> loss was observed in 54.5, 30.8 and 0.0% of grades II<\/jats:styled-content>\/III<\/jats:styled-content> astrocytomas, oligoastrocytomas and oligodendrogliomas, respectively, and 12.7% of glioblastomas. In grades II<\/jats:styled-content>\/III<\/jats:styled-content> gliomas, most ATRX<\/jats:styled-content>\u2010loss cases (92.3%) had IDH<\/jats:styled-content>1\/2<\/jats:italic> mutations. ATRX<\/jats:styled-content> loss was associated significantly with TP<\/jats:styled-content>53<\/jats:italic> mutation and p53 overexpression (P<\/jats:italic> < 0.001), but was never accompanied by 1p\/19q co\u2010deletion. IDH<\/jats:styled-content>1\/2<\/jats:italic> mutation in ATRX<\/jats:styled-content>\u2010loss tumours was less frequent in glioblastomas than in grades II<\/jats:styled-content>\/III<\/jats:styled-content> gliomas (P<\/jats:italic> < 0.001). Further, there was no significant association between ATRX<\/jats:styled-content> loss and p53 overexpression in glioblastomas. ATRX<\/jats:styled-content>\u2010loss glioblastomas affected younger patients (P<\/jats:italic> < 0.001) and occurred more frequently in locations other than the cerebral hemispheres (P<\/jats:italic> = 0.006). Most grades II<\/jats:styled-content>\/III<\/jats:styled-content> gliomas (93.3%) were categorized into three molecular subtypes based on the status of IDH<\/jats:styled-content>1\/2<\/jats:italic> mutation, ATRX<\/jats:styled-content> immunohistochemistry and 1p\/19q co\u2010deletion.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Distinct histological and molecular characteristics of adult diffuse gliomas with and without ATRX<\/jats:styled-content> immunoreactivity indicate the utility of ATRX<\/jats:styled-content> immunohistochemistry in diagnostic practice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12927","type":"journal-article","created":{"date-parts":[[2016,1,7]],"date-time":"2016-01-07T13:32:53Z","timestamp":1452173573000},"page":"260-267","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Utility of ATRX<\/scp> immunohistochemistry in diagnosis of adult diffuse gliomas"],"prefix":"10.1111","volume":"69","author":[{"given":"Masako","family":"Ikemura","sequence":"first","affiliation":[{"name":"Department of Pathology Graduate School of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junji","family":"Shibahara","sequence":"additional","affiliation":[{"name":"Department of Pathology Graduate School of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akitake","family":"Mukasa","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Graduate School of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunsaku","family":"Takayanagi","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery Graduate School of Medicine University of Tokyo Tokyo 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Graduate School of Medicine University of Tokyo Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,2,23]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Classification of the tumors of the glioma group on a histogenetic basis with a correlated study of prognosis","author":"Bailey P","year":"1926"},{"key":"e_1_2_6_3_1","volume-title":"World Health Organization classification of tumours of the central nervous system","author":"Louis 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