{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,21]],"date-time":"2025-09-21T15:26:29Z","timestamp":1758468389513},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,9,21]],"date-time":"2016-09-21T00:00:00Z","timestamp":1474416000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Orientation and digital analysis of the biliary remnants in the resected porta hepatis in infants with biliary atresia.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Samples were orientated intra\u2010operatively then stained with haematoxylin and eosin and immunostained for cytokeratin 7 (<jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>7). Sections were then digitized and analysed. Most proximal transected surface area was defined as the porta hepatis area (<jats:styled-content style=\"fixed-case\">PHA<\/jats:styled-content>) and the biliary epithelial area was defined as \u2018<jats:styled-content style=\"fixed-case\">BEA<\/jats:styled-content>\u2019. Data are quoted as median (range). Non\u2010parametric statistical comparisons were made as appropriate. <jats:italic>P<\/jats:italic> &lt; 0.05 was regarded as significant. Thirty\u2010eight infants underwent surgery [median age 53 (16\u2013120) days]. Eight specimens were excluded from the study due to technical reasons, leaving 30 specimens as the study cohort. Median <jats:styled-content style=\"fixed-case\">PHA<\/jats:styled-content> was 70 (30\u2013133) mm<jats:sup>2<\/jats:sup>, median <jats:styled-content style=\"fixed-case\">BEA<\/jats:styled-content> 0.57 (0.07\u20135.5) mm<jats:sup>2<\/jats:sup> (<jats:italic>r<\/jats:italic> = 0.51; <jats:italic>P<\/jats:italic> &lt; 0.002). The median <jats:styled-content style=\"fixed-case\">BEA<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">PHA<\/jats:styled-content> ratio was 9.6 \u00d7 10<jats:sup>\u22123<\/jats:sup> (1.9\u2013104 \u00d7 10<jats:sup>\u22123<\/jats:sup>). There was a marked correlation of <jats:styled-content style=\"fixed-case\">PHA<\/jats:styled-content> with plasma \u03b3\u2010glutamyl transpeptidase (<jats:italic>r<\/jats:italic> = \u22120.51; <jats:italic>P<\/jats:italic> = 0.001). Both total <jats:styled-content style=\"fixed-case\">BEA<\/jats:styled-content> and the <jats:styled-content style=\"fixed-case\">BEA<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">PHA<\/jats:styled-content> ratio correlated with alkaline phosphatase (<jats:italic>r<\/jats:italic> = \u22120.35; <jats:italic>P<\/jats:italic> = 0.03 and <jats:italic>r<\/jats:italic> = \u22120.47; <jats:italic>P<\/jats:italic> = 0.005, respectively). Age at surgery correlated inversely with <jats:styled-content style=\"fixed-case\">BEA<\/jats:styled-content> (<jats:italic>r<\/jats:italic> = \u22120.44; <jats:italic>P<\/jats:italic> = 0.01) but not <jats:styled-content style=\"fixed-case\">PHA<\/jats:styled-content> (<jats:italic>P<\/jats:italic> = 0.1).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Precise quantification of biliary remnants is possible and correlates with biochemical variables. Values for <jats:styled-content style=\"fixed-case\">BEA<\/jats:styled-content> were associated with and declined demonstrably with increasing age at surgery.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13028","type":"journal-article","created":{"date-parts":[[2016,7,5]],"date-time":"2016-07-05T15:59:16Z","timestamp":1467734356000},"page":"943-949","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Planimetry of the porta hepatis in biliary atresia"],"prefix":"10.1111","volume":"69","author":[{"given":"Aly","family":"Shalaby","sequence":"first","affiliation":[{"name":"Department of Paediatric Surgery King's College Hospital London UK"},{"name":"Department of Pediatric Surgery Cairo University Specialized Pediatric Hospital Cairo Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Reza","family":"Hajhosseiny","sequence":"additional","affiliation":[{"name":"Guy's &amp; St Thomas's NHS Foundation Trust London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoh","family":"Zen","sequence":"additional","affiliation":[{"name":"Institute of Liver Studies King's College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Davenport","sequence":"additional","affiliation":[{"name":"Department of Paediatric Surgery King's College Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Quaglia","sequence":"additional","affiliation":[{"name":"Institute of Liver Studies King's College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)60946-6"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)90012-3"},{"key":"e_1_2_8_4_1","first-page":"662","article-title":"Biliary atresia splenic malformation syndrome: an etiologic and prognostic subgroup","volume":"113","author":"Davenport M","year":"1993","journal-title":"Surgery"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2007.12.058"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2015.03.001"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpedsurg.2015.02.005"},{"key":"e_1_2_8_8_1","first-page":"327","article-title":"A proposal of new classification of biliary atresia","volume":"12","author":"Kasai M","year":"1976","journal-title":"J. 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