{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T07:08:23Z","timestamp":1789715303523,"version":"4.0.1"},"reference-count":39,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T00:00:00Z","timestamp":1478476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003478","name":"Ministry of Health, Labour and Welfare","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003478","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2017,2]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The aims of this study were to clarify the usefulness of immunohistochemistry in the differential diagnosis of epithelioid mesothelioma with a solid growth pattern [solid epithelioid mesothelioma (<jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content>)] and poorly differentiated squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">PDSCC<\/jats:styled-content>), and to confirm the validity of a specific type of antibody panel. Additionally, we aimed to clarify the pitfalls of immunohistochemical analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Formalin\u2010fixed paraffin\u2010embedded specimens from 36 cases of <jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content> and 38 cases of <jats:styled-content style=\"fixed-case\">PDSCC<\/jats:styled-content> were immunohistochemically examined for calretinin, podoplanin (D2\u201040), Wilms\u2019 tumour gene product (<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>1), cytokeratin (<jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>) 5\/6, p40, p63, carcinoembryonic antigen (<jats:styled-content style=\"fixed-case\">CEA<\/jats:styled-content>), epithelial\u2010related antigen (<jats:styled-content style=\"fixed-case\">MOC<\/jats:styled-content>31), claudin\u20104, thyroid transcription factor\u20101 (<jats:styled-content style=\"fixed-case\">TTF<\/jats:styled-content>\u20101), and napsin A. <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>1 showed the highest diagnostic accuracy (85.1%) as a mesothelial marker, and <jats:styled-content style=\"fixed-case\">CEA<\/jats:styled-content>, p40 and claudin\u20104 showed higher diagnostic accuracies (95.9%, 94.6%, and 93.2%, respectively) as carcinoma markers. Calretinin (diagnostic accuracy: 75.7%), D2\u201040 (diagnostic accuracy: 67.6%), <jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>5\/6 (diagnostic accuracy: 63.5%), <jats:styled-content style=\"fixed-case\">TTF<\/jats:styled-content>\u20101 (diagnostic accuracy: 55.4%) and napsin A (diagnostic accuracy: 52.7%) could not differentiate between <jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PDSCC<\/jats:styled-content>. Among these markers, the combination of calretinin and <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>1 showed the highest diagnostic accuracy (86.5%) as a positive marker, and the combination of p40 and <jats:styled-content style=\"fixed-case\">CEA<\/jats:styled-content> showed the highest diagnostic accuracy (97.3%) as a negative marker. The combination of <jats:styled-content style=\"fixed-case\">CEA<\/jats:styled-content> and claudin\u20104 also showed relatively high diagnostic accuracy (94.6%) as a negative marker.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We recommend the combination of <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>1 and calretinin as a positive maker, and the combination of <jats:styled-content style=\"fixed-case\">CEA<\/jats:styled-content> and claudin\u20104 as a negative marker, for differential diagnoses of <jats:styled-content style=\"fixed-case\">SEM<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PDSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13073","type":"journal-article","created":{"date-parts":[[2016,9,2]],"date-time":"2016-09-02T16:45:29Z","timestamp":1472834729000},"page":"375-384","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Utility and pitfalls of immunohistochemistry in the differential diagnosis between epithelioid mesothelioma and poorly differentiated lung squamous cell carcinoma"],"prefix":"10.1111","volume":"70","author":[{"given":"Kei","family":"Kushitani","sequence":"first","affiliation":[{"name":"Department of Pathology Institute of Biomedical and Health Sciences Hiroshima University Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4986-2109","authenticated-orcid":false,"given":"Vishwa J","family":"Amatya","sequence":"additional","affiliation":[{"name":"Department of Pathology Institute of Biomedical and Health Sciences Hiroshima University Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuko","family":"Okada","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Hiroshima University Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuya","family":"Katayama","sequence":"additional","affiliation":[{"name":"Faculty of Medicine Hiroshima University Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amany S","family":"Mawas","sequence":"additional","affiliation":[{"name":"Department of Pathology Institute of Biomedical and Health Sciences Hiroshima University Hiroshima Japan"},{"name":"Department of Pathology and Clinical Pathology Faculty of Veterinary Medicine South Valley University Qena Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiro","family":"Miyata","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology Research Centre for Radiation Casualty Medicine Research Institute for Radiation Biology and Medicine Hiroshima University Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morihito","family":"Okada","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology Research Centre for Radiation Casualty Medicine Research Institute for Radiation Biology and Medicine Hiroshima University Hiroshima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouki","family":"Inai","sequence":"additional","affiliation":[{"name":"Department of Pathology Institute of Biomedical and Health Sciences Hiroshima University Hiroshima Japan"},{"name":"Pathologic Diagnostic Centre, Inc. 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