{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T09:21:18Z","timestamp":1759742478645,"version":"3.37.3"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,1,9]],"date-time":"2017-01-09T00:00:00Z","timestamp":1483920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472391","81372743"],"award-info":[{"award-number":["81472391","81372743"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The aim of this study was to investigate potential molecular mechanisms associated with loss of <jats:styled-content style=\"fixed-case\">BRM<\/jats:styled-content> expression in poorly differentiated clear cell renal cell carcinoma (cc<jats:styled-content style=\"fixed-case\">RCC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Nineteen previously selected <jats:styled-content style=\"fixed-case\">BRM<\/jats:styled-content>\u2010negative <jats:styled-content style=\"fixed-case\">RCC<\/jats:styled-content> tissues were examined by <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> sequencing, fluorescence <jats:italic>in\u2010situ<\/jats:italic> hybridization (<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>) and methylation\u2010specific polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) of the <jats:italic><jats:styled-content style=\"fixed-case\">BRM<\/jats:styled-content><\/jats:italic> gene. <jats:italic><jats:styled-content style=\"fixed-case\">BRM<\/jats:styled-content><\/jats:italic> mutation was identified in 78.9% (15 of 19) cases, chromosome 9 monosomy or <jats:italic><jats:styled-content style=\"fixed-case\">BRM<\/jats:styled-content><\/jats:italic> deletion in 43.8% (seven of 16) and <jats:italic><jats:styled-content style=\"fixed-case\">BRM<\/jats:styled-content><\/jats:italic> promoter region cytosine\u2013phosphate\u2013guanine (CpG) methylation in 42.8% (six of 14). These results indicated that 89.5% (17 of 19) of the cases harboured at least one type of <jats:italic><jats:styled-content style=\"fixed-case\">BRM<\/jats:styled-content><\/jats:italic> genetic alteration, with two or more types of alteration in 47.4% (nine of 19). Such alterations were found rarely in adjacent non\u2010neoplastic tissues and low\u2010grade areas of composite tumours.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">BRM<\/jats:styled-content><\/jats:italic> gene mutation, chromosome 9 monosomy or <jats:italic><jats:styled-content style=\"fixed-case\">BRM<\/jats:styled-content><\/jats:italic> deletion and CpG methylation contribute collectively to the loss of <jats:styled-content style=\"fixed-case\">BRM<\/jats:styled-content> expression in cc<jats:styled-content style=\"fixed-case\">RCC<\/jats:styled-content>. This work focusing on composite tumours indicated that <jats:italic><jats:styled-content style=\"fixed-case\">BRM<\/jats:styled-content><\/jats:italic> abnormality occurred during tumour progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13120","type":"journal-article","created":{"date-parts":[[2016,11,7]],"date-time":"2016-11-07T15:32:34Z","timestamp":1478532754000},"page":"711-721","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<scp>BRM<\/scp>\/<scp>SMARCA<\/scp>2\u2010negative clear cell renal cell carcinoma is associated with a high percentage of <i><scp>BRM<\/scp><\/i> somatic mutations, deletions and promoter methylation"],"prefix":"10.1111","volume":"70","author":[{"given":"Qiu\u2010Yuan","family":"Xia","sequence":"first","affiliation":[{"name":"Department of Pathology Nanjing Jinling Hospital Nanjing University School of Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue\u2010Mei","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Pathology Linyi People's Hospital Linyi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang\u2010Shan","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Pathology Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Nanjing City Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheng\u2010Bing","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Pathology Nanjing Jinling Hospital Nanjing University School of Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan\u2010Shan","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Pathology Nanjing Jinling Hospital Nanjing University School of Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology Nanjing Jinling Hospital Nanjing University School of Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pathology Nanjing Jinling Hospital Nanjing University School of Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology Nanjing Jinling Hospital Nanjing University School of Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng\u2010Hui","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Pathology Nanjing Jinling Hospital Nanjing University School of Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen\u2010Feng","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pathology Nanjing Jinling Hospital Nanjing University School of Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao\u2010Jun","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Pathology Nanjing Jinling Hospital Nanjing University School of Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2674-1937","authenticated-orcid":false,"given":"Qiu","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Pathology Nanjing Jinling Hospital Nanjing University School of Medicine Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,1,9]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"14","volume-title":"WHO classification of tumours of the urinary system and male genital organs (IARC WHO Classification of Tumours)","author":"Moch H","year":"2016"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2708"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.seminoncol.2013.05.006"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31825aafb5"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000502"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31827e17cb"},{"key":"e_1_2_6_8_1","first-page":"29","article-title":"Molecular genetics and immunohistochemistry characterization of uncommon and recently described renal cell carcinomas","volume":"28","author":"Rao Q","year":"2016","journal-title":"Chin. 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