{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:30:21Z","timestamp":1790173821324,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,8,8]],"date-time":"2017-08-08T00:00:00Z","timestamp":1502150400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"RFO University of Bologna, Italy"},{"name":"Associazione Italiana Leucemie, Linfomi e Mielomi (AIL-Bologna)"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2017,11]]},"abstract":"Aims<\/jats:title>Mantle cell lymphoma (MCL<\/jats:styled-content>) is characterized by distinctive histological and molecular features. Aberrant expression of BCL<\/jats:styled-content>6 and CD<\/jats:styled-content>10 has been reported occasionally, but the biological features of such cases are largely unknown. This study aimed to define the epidemiological, histological and cytogenetic characteristics of BCL<\/jats:styled-content>6 and CD<\/jats:styled-content>10\u2010positive MCL<\/jats:styled-content>s, also investigating possible biological features.<\/jats:p><\/jats:sec>Methods and results<\/jats:title>A total of 165 cases of cyclin D1 and t(11;14)(q13;q34)\u2010positive MCL<\/jats:styled-content>s were studied for CD<\/jats:styled-content>10 and BCL<\/jats:styled-content>6 immunohistochemical expression, which was documented in 26 of 165 (15.8%) cases (BCL<\/jats:styled-content>6 17 of 165; CD<\/jats:styled-content>10 11 of 165; BCL<\/jats:styled-content>6 and CD<\/jats:styled-content>10 co\u2010expression two of 165). CD<\/jats:styled-content>10\u2010positivity was significantly more frequent in females (63.3%; P<\/jats:italic> < 0.01). Either expression correlated significantly with higher mean proliferation index and higher prevalence of MUM<\/jats:styled-content>1 positivity (P<\/jats:italic> < 0.05). Fluorescence in\u2010situ<\/jats:italic> hybridization (FISH<\/jats:styled-content>) for BCL<\/jats:styled-content>6<\/jats:italic> (3q27) gene derangements was performed on the BCL<\/jats:styled-content>6\u2010 and CD<\/jats:styled-content>10\u2010positive cases and 98 matched controls: amplifications were documented more frequently in BCL<\/jats:styled-content>6\u2010positive than \u2010negative cases (50.0% versus 19.4% of cases) (P<\/jats:italic> < 0.05). The mutational status of the variable immunoglobulin heavy chain genes (IGVH<\/jats:styled-content><\/jats:italic>) was investigated by Sanger sequencing: five of the six successfully tested cases (83.3%) showed no somatic hypermutations.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Aberrant CD<\/jats:styled-content>10 and BCL<\/jats:styled-content>6 expression defines a subset of MCL<\/jats:styled-content>s with higher mean Ki\u201067 index and higher prevalence of MUM<\/jats:styled-content>1 expression. BCL<\/jats:styled-content>6 protein positivity correlates with cytogenetic aberrations involving the BCL<\/jats:styled-content>6<\/jats:italic> gene. Although examined successfully in few cases, the high prevalence of unmutated IGVH<\/jats:styled-content><\/jats:italic> genes also points at a pregerminal cell origin for these phenotypically aberrant cases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13286","type":"journal-article","created":{"date-parts":[[2017,6,19]],"date-time":"2017-06-19T14:25:22Z","timestamp":1497882322000},"page":"769-777","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Aberrant expression of CD<\/scp>10 and BCL<\/scp>6 in mantle cell lymphoma"],"prefix":"10.1111","volume":"71","author":[{"given":"Marco","family":"Pizzi","sequence":"first","affiliation":[{"name":"Surgical Pathology and Cytopathology Unit Department of Medicine\u2010DIMED University of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4945-1422","authenticated-orcid":false,"given":"Claudio","family":"Agostinelli","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department of Hematology and Oncology\/Department of Experimental Diagnostic and Specialty Medicine Sant'Orsola University Hospital Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Righi","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department of Hematology and Oncology\/Department of Experimental Diagnostic and Specialty Medicine Sant'Orsola University Hospital Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Gazzola","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department of Hematology and Oncology\/Department of Experimental Diagnostic and Specialty Medicine Sant'Orsola University Hospital Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Mannu","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department of Hematology and Oncology\/Department of Experimental Diagnostic and Specialty Medicine Sant'Orsola University Hospital Bologna Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Galuppini","sequence":"additional","affiliation":[{"name":"Surgical Pathology and Cytopathology Unit Department of Medicine\u2010DIMED University of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6515-5482","authenticated-orcid":false,"given":"Matteo","family":"Fassan","sequence":"additional","affiliation":[{"name":"Surgical Pathology and Cytopathology Unit Department of Medicine\u2010DIMED University of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Visentin","sequence":"additional","affiliation":[{"name":"Hematology and Clinical Immunology Unit Department of Medicine\u2010DIMED University of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Piazza","sequence":"additional","affiliation":[{"name":"Hematology and Clinical Immunology Unit Department of Medicine\u2010DIMED University of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianpietro C","family":"Semenzato","sequence":"additional","affiliation":[{"name":"Hematology and Clinical Immunology Unit Department of Medicine\u2010DIMED University of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Rugge","sequence":"additional","affiliation":[{"name":"Surgical Pathology and Cytopathology Unit Department of Medicine\u2010DIMED University of Padova Padova Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4136-3591","authenticated-orcid":false,"given":"Elena","family":"Sabattini","sequence":"additional","affiliation":[{"name":"Haematopathology Unit Department of Hematology and Oncology\/Department of Experimental Diagnostic and Specialty Medicine Sant'Orsola University Hospital Bologna 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