{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:22:40Z","timestamp":1740126160082,"version":"3.37.3"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,10]],"date-time":"2017-10-10T00:00:00Z","timestamp":1507593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>In the near future, an immunoscore based on the quantification of lymphocytic populations can be expected as a fundamental supplement of colorectal cancer (<jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content>) classification. This study explored whether latent viral infection has an influence on prognostically relevant host immunity in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> lymphocytic infiltration in three tumour compartments of 121 <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> was compared with clinical data and occurrence of latent infection with herpes simplex virus (HSV1, HSV2), cytomegalovirus (<jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content>), human papillomavirus (HPV16 and HPV18) in the tumour tissue, which was determined by polymerase chain reaction (<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>). Intraepithelial <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> lymphocytic infiltration (<jats:styled-content style=\"fixed-case\">IE<jats:sub>CD<\/jats:sub><\/jats:styled-content><jats:sub>8+<\/jats:sub>) showed a trend towards correlation with clinical stage (<jats:italic>P<\/jats:italic> = 0.073), significant differences between <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> with and without metastases (<jats:italic>P<\/jats:italic> = 0.001) and a significant correlation with overall survival (<jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content>,<jats:italic> P<\/jats:italic> = 0.001). Each of these three clinical parameters showed a significant link to <jats:styled-content style=\"fixed-case\">IE<jats:sub>CD<\/jats:sub><\/jats:styled-content><jats:sub>8+<\/jats:sub> in the virus <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010negative (<jats:italic>P<\/jats:italic>\u2010values: 0.001\u20130.036), but no significant differences in the virus <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010positive subgroup, which is consistent with a moderating effect of virus <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> on these associations. A significant correlation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> infiltration in the invasive margin (<jats:styled-content style=\"fixed-case\">IM<jats:sub>CD<\/jats:sub><\/jats:styled-content><jats:sub>8+<\/jats:sub>) with <jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content> (<jats:italic>P<\/jats:italic> = 0.016) was also moderated by virus <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data suggest a possible influence of latent viral infection on the association between clinical outcome and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> lymphocytic infiltration in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> tissue. After confirmation of these results by large cohort studies, a potential interaction between microbial pathogens and host immunity in <jats:styled-content style=\"fixed-case\">CRC<\/jats:styled-content> and its impact on prognostic immunoscores and\/or new therapeutic strategies should be investigated further.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13325","type":"journal-article","created":{"date-parts":[[2017,7,26]],"date-time":"2017-07-26T22:32:54Z","timestamp":1501108374000},"page":"201-215","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The association between clinical outcome and <scp>CD<\/scp>8<sup>+<\/sup> lymphocytic infiltration in advanced stages of colorectal cancer differs by latent virus infection in tumour tissue"],"prefix":"10.1111","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1343-3422","authenticated-orcid":false,"given":"Britta","family":"Kleist","sequence":"first","affiliation":[{"name":"Department of Pathology Southern Hospital Trust Kristiansand Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marius","family":"Bagdonas","sequence":"additional","affiliation":[{"name":"Department of Pathology Southern Hospital Trust Kristiansand Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prakash","family":"Oommen","sequence":"additional","affiliation":[{"name":"Department of Pathology Southern Hospital Trust Kristiansand Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irina","family":"Schoenhardt","sequence":"additional","affiliation":[{"name":"Department of Pathology Southern Hospital Trust Kristiansand Norway"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janina","family":"Levermann","sequence":"additional","affiliation":[{"name":"Institute of Legal Medicine University Hospital Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Micaela","family":"Poetsch","sequence":"additional","affiliation":[{"name":"Institute of Legal Medicine University Hospital Essen Essen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.29210"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.08.2644"},{"volume-title":"TNM classification of malign tumours","year":"2009","author":"Sobin LH","key":"e_1_2_7_4_1"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)17706-X"},{"volume-title":"World Health Organization classification of tumors. 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