{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T22:45:05Z","timestamp":1782254705213,"version":"3.54.5"},"reference-count":36,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,28]],"date-time":"2018-02-28T00:00:00Z","timestamp":1519776000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Interobserver agreement for dysplasia in Barrett's oesophagus (BO) is low, and guidelines advise expert review of dysplastic cases. The aim of this study was to assess the added value of p53 immunohistochemistry (IHC) for the homogeneity within a group of dedicated gastrointestinal (GI) pathologists.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Sixty\u2010single haematoxylin and eosin (HE) slide referral BO cases [20 low\u2010grade dysplasia (LGD); 20 high\u2010grade dysplasia (HGD); and 20 non\u2010dysplastic BO reference cases] were digitalised and independently assessed twice in random order by 10 dedicated GI pathologists. After a \u2018wash\u2010out\u2019 period, cases were reassessed with the addition of a corresponding p53 IHC slide. Outcomes were: (i) proportion of \u2018indefinite for dysplasia\u2019 (IND) diagnoses; (ii) interobserver agreement; and (iii) diagnostic accuracy as compared with a consensus \u2018gold standard\u2019 diagnosis defined at an earlier stage by five core expert BO pathologists after their assessment of this case set. Addition of p53 IHC decreased the mean proportion of IND diagnoses from 10 of 60 to eight of 60 (<jats:italic>P<\/jats:italic> = 0.071). Mean interobserver agreement increased significantly from 0.45 to 0.57 (<jats:italic>P<\/jats:italic> = 0.0021). The mean diagnostic accuracy increased significantly from 72% to 82% (<jats:italic>P<\/jats:italic> = 0.0072) after p53 IHC addition.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Addition of p53 IHC significantly improves the histological assessment of BO biopsies, even within a group of dedicated GI pathologists. It decreases the proportion of IND diagnoses, and increases interobserver agreement and diagnostic accuracy. This justifies the use of accessory p53 IHC within our upcoming national digital review panel for BO biopsy cases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13462","type":"journal-article","created":{"date-parts":[[2018,1,3]],"date-time":"2018-01-03T16:11:48Z","timestamp":1514995908000},"page":"1015-1023","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Improved diagnostic stratification of digitised Barrett's oesophagus biopsies by p53 immunohistochemical staining"],"prefix":"10.1111","volume":"72","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5825-3236","authenticated-orcid":false,"given":"Myrtle J","family":"van der Wel","sequence":"first","affiliation":[{"name":"Department of Pathology Academic Medical Centre Amsterdam  The Netherlands"},{"name":"Department of Gastroenterology and Hepatology Academic Medical Centre Amsterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucas C","family":"Duits","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Academic Medical Centre Amsterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roos E","family":"Pouw","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Academic Medical Centre Amsterdam  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelis A","family":"Seldenrijk","sequence":"additional","affiliation":[{"name":"Department of Pathology Pathology\u2010DNA St Antonius Hospital Nieuwegein  The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G J A","family":"Offerhaus","sequence":"additional","affiliation":[{"name":"Department of Pathology University Medical Centre Utrecht  The 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