{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T14:05:34Z","timestamp":1785679534916,"version":"3.56.0"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,2,26]],"date-time":"2018-02-26T00:00:00Z","timestamp":1519603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100005699","name":"Universit\u00e0 degli Studi del Piemonte Orientale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005699","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>High mobility group box 1 (<jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1) is a chromatin structural protein, expressed ubiquitously in the nuclei of mammalian cells. When transported extracellularly, it acts as a tumour suppressor and oncogenic protein. In malignant pleural mesothelioma (<jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content>), high serum levels of <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 have been related to a poor prognosis. Conversely, the significance of <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 expression in <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content> tissues is still unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Biopsy samples from 170 patients with <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content> were assessed by immunohistochemistry and reverse transcription\u2013polymerase chain reaction (<jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) to evaluate <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 protein and gene expression. The expression level of <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 protein was scored using a semiquantitative system that sums the intensity (0\u20133) and the percentage (from 0 to 4) of positively stained cells in nuclei, cytoplasm and in both. The final score was considered as high (&gt;3) or low (&lt;3) expression. Gene expression levels were calculated using the \u0394\u0394C<jats:sub>t<\/jats:sub> method. High expression levels of <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 as total (<jats:italic>P<\/jats:italic> = 0.0011) and cytoplasmic score (<jats:italic>P<\/jats:italic> = 0.0462) were related to a worse disease\u2010specific survival (<jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content>) in the entire cohort and in the clinicopathological subgroups. No significant correlation was found between <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 gene expression and <jats:styled-content style=\"fixed-case\">DSS<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings indicate that <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 may be a useful prognostic biomarker in <jats:styled-content style=\"fixed-case\">MPM<\/jats:styled-content> when detected by immunohistochemistry. Conversely, as it is also expressed in normal and reactive mesothelial cells, <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 cannot be considered a diagnostic biomarker in histological samples of mesothelioma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13470","type":"journal-article","created":{"date-parts":[[2018,1,22]],"date-time":"2018-01-22T14:58:28Z","timestamp":1516633108000},"page":"1039-1050","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Expression analysis of <scp>HMGB<\/scp>1 in histological samples of malignant pleural mesothelioma"],"prefix":"10.1111","volume":"72","author":[{"given":"Eltjona","family":"Rrapaj","sequence":"first","affiliation":[{"name":"Department of Health Sciences University of Eastern Piedmont Novara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elena","family":"Trisolini","sequence":"additional","affiliation":[{"name":"Department of Health Sciences University of Eastern Piedmont Novara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luca","family":"Bertero","sequence":"additional","affiliation":[{"name":"Division of Pathology Department of Medical Sciences University of Turin Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michela","family":"Salvo","sequence":"additional","affiliation":[{"name":"Department of Health Sciences University of Eastern Piedmont Novara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rossella","family":"Indellicato","sequence":"additional","affiliation":[{"name":"Department of Health Sciences University of Eastern Piedmont Novara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvano","family":"Andorno","sequence":"additional","affiliation":[{"name":"Department of Health Sciences University of Eastern Piedmont Novara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose M","family":"Garcia\u2010Manteiga","sequence":"additional","affiliation":[{"name":"Center For Translational Genomics and Bioinformatics IRCCS San Raffaele Hospital Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ottavio","family":"Rena","sequence":"additional","affiliation":[{"name":"Unit of Thoracic Surgery Maggiore della Carit\u00e0 Hospital Novara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1183-2737","authenticated-orcid":false,"given":"Renzo L","family":"Boldorini","sequence":"additional","affiliation":[{"name":"Department of Health Sciences University of Eastern Piedmont Novara Italy"},{"name":"Unit of Pathology Maggiore della Carit\u00e0 Hospital Novara Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,2,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/014107680509801010"},{"key":"e_1_2_7_3_1","volume-title":"WHO Classification of tumours of the lung, pleura, thymus and heart","author":"Travis W","year":"2015"},{"key":"e_1_2_7_4_1","first-page":"32","article-title":"Peritoneal mesothelioma: a review","volume":"9","author":"Bridda A","year":"2007","journal-title":"MedGenMed."},{"key":"e_1_2_7_5_1","first-page":"260","article-title":"Diffuse pleural mesothelioma and asbestos exposure in the North Western Cape Province","volume":"17","author":"Wagner JC","year":"1960","journal-title":"Br. J. Ind. Med."},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11864-008-0067-z"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.912"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6690105"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2012-0214-OA"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncponc1039"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.11.136"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.1.145"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvd030"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/20.16.4337"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1594"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-009-0305-0"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070679"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2010.94"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.02-0621fje"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2004.062729"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2391"},{"key":"e_1_2_7_22_1","first-page":"449","article-title":"Expression of high mobility group box chromosomal protein\u20101 (HMGB\u20101) in gastric cancer","volume":"27","author":"Akaike H","year":"2007","journal-title":"Anticancer Res."},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2010.07.016"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1006542107"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-3481"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0b013e318297fa65"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-13-205"},{"key":"e_1_2_7_28_1","volume-title":"TNM classification of malignant tumours","author":"Brierley JD","year":"2016"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000421-198212000-00014"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0653"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-7-25"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22724"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/1750-1172-3-34"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.39.6671"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa051185"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1115050"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.10413"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-13-0495"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvf198"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200911078"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2003.01296.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-1953"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2009.11.014"},{"key":"e_1_2_7_45_1","first-page":"5357","article-title":"Prognostic significance of high mobility group box 1 (HMGB1) expression in patients with colorectal cancer","volume":"34","author":"Ueda M","year":"2014","journal-title":"Anticancer Res."},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.4161\/auto.27418"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmt109"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/joim.12309"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-15-1130"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhis.13470","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.13470","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T16:09:02Z","timestamp":1695571742000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.13470"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,26]]},"references-count":48,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1111\/his.13470"],"URL":"https:\/\/doi.org\/10.1111\/his.13470","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,26]]},"assertion":[{"value":"2017-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-01-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}