{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T12:42:20Z","timestamp":1780922540926,"version":"3.54.1"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2018,3,9]],"date-time":"2018-03-09T00:00:00Z","timestamp":1520553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The programmed death 1 (<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>1)\/<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>1 ligand (<jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1) axis plays an important role in tumour cells escape from immune control. <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 immunohistochemistry is a useful predictor of immunotherapy response, but is still not used widely in the diagnostic setting. Here we describe results using <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 immunohistochemistry during routine diagnostics in lymphoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Ninety\u2010one lymphoproliferative disease cases sharing tumour and non\u2010malignant Hodgkin\u2013Reed\u2010Sternberg (<jats:styled-content style=\"fixed-case\">HRS<\/jats:styled-content>)\u2010like cells with and without Epstein\u2013Barr virus (<jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content>) association were investigated by immunohistochemistry for <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 (clone <jats:styled-content style=\"fixed-case\">SP<\/jats:styled-content>142). <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 expression was present in more than 5% of tumour or non\u2010malignant <jats:styled-content style=\"fixed-case\">HRS<\/jats:styled-content>\u2010like cells in 100% of <jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content><jats:sup>+<\/jats:sup> classical (C) Hodgkin lymphoma (<jats:styled-content style=\"fixed-case\">HL<\/jats:styled-content>) (<jats:italic>n<\/jats:italic>\u00a0=\u00a010) and <jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content>\u2010negative nodular sclerosis <jats:styled-content style=\"fixed-case\">CHL<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a08); 40% of <jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content><jats:sup>+<\/jats:sup> diffuse large B cell lymphoma, not otherwise specified (<jats:styled-content style=\"fixed-case\">DLBCL<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content>) (<jats:italic>n<\/jats:italic>\u00a0=\u00a020); and 4% of nodal peripheral T cell lymphoma of follicular helper T cell type (<jats:styled-content style=\"fixed-case\">PTCL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TFH<\/jats:styled-content>) (<jats:italic>n<\/jats:italic>\u00a0=\u00a022). In contrast, nodular lymphocyte\u2010predominant <jats:styled-content style=\"fixed-case\">HL<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a04), lymphocyte\u2010rich <jats:styled-content style=\"fixed-case\">CHL<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u00a0=\u00a06), <jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content><jats:sup>+<\/jats:sup> hyperplasia (<jats:italic>n<\/jats:italic>\u00a0=\u00a08), plasmablastic lymphoma (<jats:italic>n<\/jats:italic>\u00a0= 3) and anaplastic lymphoma kinase\u2010negative anaplastic large cell lymphoma (<jats:italic>n<\/jats:italic>\u00a0=\u00a05) seldom exhibited <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 in their large cells. Assessing <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 positivity in tumour and non\u2010malignant large cells was helpful in differentiating between <jats:styled-content style=\"fixed-case\">CHL<\/jats:styled-content> versus nodal <jats:styled-content style=\"fixed-case\">PTCL<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">TFH<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.0001) or <jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content><jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">DLBCL<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0052) and between <jats:styled-content style=\"fixed-case\">EBV<\/jats:styled-content><jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">DLBCL<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content> versus nodal <jats:styled-content style=\"fixed-case\">PTCL<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">TFH<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.0052), with <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 expression indicating the first diagnosis in each of those sets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Immunohistochemical evaluation of <jats:styled-content style=\"fixed-case\">PD<\/jats:styled-content>\u2010L1 expression in tumour and non\u2010malignant <jats:styled-content style=\"fixed-case\">HRS<\/jats:styled-content>\u2010like large cells may be useful for assessing either immune escape or immunodeficiency in their pathogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13475","type":"journal-article","created":{"date-parts":[[2018,1,30]],"date-time":"2018-01-30T07:18:28Z","timestamp":1517296708000},"page":"1156-1163","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Immunohistochemical assessment of the diagnostic utility of <scp>PD<\/scp>\u2010L1: a preliminary analysis of anti\u2010<scp>PD<\/scp>\u2010L1 antibody (<scp>SP<\/scp>142) for lymphoproliferative diseases with tumour and non\u2010malignant Hodgkin\u2013Reed\u2010Sternberg (<scp>HRS<\/scp>)\u2010like cells"],"prefix":"10.1111","volume":"72","author":[{"given":"Ayako","family":"Sakakibara","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Nagoya University Hospital Nagoya Japan"},{"name":"Department of Pathology Okazaki City Hospital Okazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kei","family":"Kohno","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Nagoya University Hospital Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed E","family":"Eladl","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Nagoya University Hospital Nagoya Japan"},{"name":"Department of Pathology Faculty of Medicine Mansoura University Mansoura Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5968-218X","authenticated-orcid":false,"given":"Teerada","family":"Klaisuwan","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Nagoya University Hospital Nagoya Japan"},{"name":"Department of Pathology Faculty of Medicine Chiang Mai University Chiang Mai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eri","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Nagoya University Hospital Nagoya Japan"},{"name":"Department of Gastroenterology and Hepatology Aichi Medical University Hospital Nagakute Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2288-7162","authenticated-orcid":false,"given":"Yuka","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Nagoya University Hospital Nagoya Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoko","family":"Shimada","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Nagoya University Hospital Nagoya 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