{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T23:14:16Z","timestamp":1784848456528,"version":"3.55.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,4,19]],"date-time":"2018-04-19T00:00:00Z","timestamp":1524096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P50 CA174521\u201001A1"],"award-info":[{"award-number":["P50 CA174521\u201001A1"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2018,7]]},"abstract":"Aims<\/jats:title>Merkel cell carcinoma represents poorly differentiated neuroendocrine carcinoma of cutaneous origin. In most studies, the vast majority of Merkel cell carcinomas are Merkel cell polyomavirus (MCP<\/jats:styled-content>yV)\u2010associated. SV<\/jats:styled-content>40 polyomavirus immunohistochemistry is typically used in the diagnosis of other polyomavirus\u2010associated diseases, including tubulointerstitial nephritis and progressive multifocal leukoencephalopathy, given cross\u2010reactivity with BK<\/jats:styled-content> and JC<\/jats:styled-content> polyomaviruses. MCP<\/jats:styled-content>yV\u2010specific immunohistochemistry is commercially available, but, if antibodies against SV<\/jats:styled-content>40 also cross\u2010reacted with MCP<\/jats:styled-content>yV, that would be advantageous from a resource\u2010utilisation perspective.<\/jats:p><\/jats:sec>Methods and results<\/jats:title>Tissue microarrays were constructed from 39 Merkel cell carcinomas, 24 small\u2010cell lung carcinomas, and 18 extrapulmonary visceral small\u2010cell carcinomas. SV<\/jats:styled-content>40 large T antigen immunohistochemistry (clone PA<\/jats:styled-content>b416) was performed; MCP<\/jats:styled-content>yV large T antigen immunohistochemistry (clone CM<\/jats:styled-content>2B4) had been previously performed. UniProt was used to compare the amino acid sequences of the SV<\/jats:styled-content>40, BK<\/jats:styled-content>, JC<\/jats:styled-content> and MCPyV large T antigens, focusing on areas recognised by the PA<\/jats:styled-content>b416 and CM<\/jats:styled-content>2B4 clones. SV<\/jats:styled-content>40 immunohistochemistry was negative in all tumours; MCP<\/jats:styled-content>yV immunohistochemistry was positive in 38% of Merkel cell carcinomas and in 0% of non\u2010cutaneous poorly differentiated neuroendocrine carcinomas. UniProt analysis revealed a high degree of similarity between SV<\/jats:styled-content>40, BK<\/jats:styled-content>, and JC<\/jats:styled-content> viruses in the region recognised by PA<\/jats:styled-content>b416. There was less homology between SV<\/jats:styled-content>40 and MCP<\/jats:styled-content>yV in this region, which was also interrupted by two long stretches of amino acids unique to MCP<\/jats:styled-content>yV. The CM<\/jats:styled-content>2B4 clone recognises a unique epitope in one of these stretches.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The PAb416 antibody against the SV<\/jats:styled-content>40 large T antigen does not cross\u2010react with MCP<\/jats:styled-content>yV large T antigen, and thus does not label Merkel cell carcinoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13483","type":"journal-article","created":{"date-parts":[[2018,2,11]],"date-time":"2018-02-11T11:06:35Z","timestamp":1518347195000},"page":"162-166","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["A monoclonal antibody against SV<\/scp>40 large T antigen (PA<\/scp>b416) does not label Merkel cell carcinoma"],"prefix":"10.1111","volume":"73","author":[{"given":"Daniel J","family":"Pelletier","sequence":"first","affiliation":[{"name":"Department of Pathology University of Iowa Hospitals and Clinics and Carver College of Medicine Iowa City IA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas W","family":"Czeczok","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8264-6379","authenticated-orcid":false,"given":"Andrew M","family":"Bellizzi","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Iowa Hospitals and Clinics and Carver College of Medicine Iowa City IA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,4,19]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archderm.1972.01620040075020"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197811)42:5<2311::AID-CNCR2820420531>3.0.CO;2-L"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0560.2009.01370.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jid.5700925"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1152586"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0806526105"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24510"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAP.0b013e3182a2dc67"},{"key":"e_1_2_9_10_1","unstructured":"http:\/\/nationalaglawcenter.org\/wp-content\/uploads\/assets\/OALJdecisions\/consent\/05192016_AWA_Dockets-12-0536-15-0023-15-0165_Santa-Cruz-Biotechnology-Inc.pdf"},{"issue":"2","key":"e_1_2_9_11_1","first-page":"132A","article-title":"Immunohistochemistry for Merkel cell polyomavirus large T antigen (CM2B4) and neurofilament protein adds little value to CK20 and TTF\u20101 in the distinction of cutaneous and visceral high\u2010grade neuroendocrine carcinomas","volume":"27","author":"Czeczok TW","year":"2014","journal-title":"Mod. 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