{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T23:31:29Z","timestamp":1785367889289,"version":"3.55.0"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,5,30]],"date-time":"2018-05-30T00:00:00Z","timestamp":1527638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Pseudosarcomatous myofibroblastic proliferations of the genitourinary tract have a debatable relationship with inflammatory myofibroblastic tumour (generally lacking <jats:italic>ALK<\/jats:italic> rearrangement); however, they share several overlapping features with nodular fasciitis of soft tissue. As rearrangement of the USP6 gene has been recently recognised as a recurrent alteration in soft tissue nodular fasciitis, and several other alternative gene fusions have been recently recognised in inflammatory myofibroblastic tumour, the aim of this study was to investigate whether <jats:italic>USP6<\/jats:italic>,<jats:italic> ROS1<\/jats:italic> or <jats:italic>ETV6<\/jats:italic> rearrangements were present in these lesions (12 cases).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Fluorescence <jats:italic>in\u2010situ<\/jats:italic> hybridisation analysis was performed by the use of bacterial artificial chromosome\u2010derived break\u2010apart probes against <jats:italic>USP6<\/jats:italic>,<jats:italic> ROS1<\/jats:italic>, and <jats:italic>ETV6<\/jats:italic>. Two cases with adequate genetic material from recent paraffin tissue blocks were also tested by use of a solid tumour gene fusion detection assay via next\u2010generation sequencing, targeting &gt;50 known genes involved in recurrent fusions. None of the genitourinary pseudosarcomatous myofibroblastic proliferations was found to harbour <jats:italic>USP6<\/jats:italic> (0\/12), <jats:italic>ROS1<\/jats:italic> (0\/8) or <jats:italic>ETV6<\/jats:italic> (0\/7) rearrangements, and no gene fusions were detected in two cases studied by sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Despite overlap in histological and immunohistochemical features between pseudosarcomatous myofibroblastic proliferation and nodular fasciitis, these tumours lack the recently recognised <jats:italic>USP6<\/jats:italic> rearrangements that occur in nodular fasciitis, as well as alternative fusions found in <jats:italic>ALK\u2010<\/jats:italic>negative inflammatory myofibroblastic tumours. At present, this diagnosis remains based primarily on clinical, histological and immunohistochemical features.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13526","type":"journal-article","created":{"date-parts":[[2018,4,4]],"date-time":"2018-04-04T14:50:52Z","timestamp":1522853452000},"page":"321-326","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Pseudosarcomatous myofibroblastic proliferations of the genitourinary tract are genetically different from nodular fasciitis and lack <i>USP6<\/i>,<i> ROS1<\/i> and <i>ETV6<\/i> gene rearrangements"],"prefix":"10.1111","volume":"73","author":[{"given":"Judith A S","family":"Jebastin","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine and Henry Ford Cancer Institute Henry Ford Health System Detroit MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven 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