{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T04:17:02Z","timestamp":1790223422102,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,7,27]],"date-time":"2018-07-27T00:00:00Z","timestamp":1532649600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["P30 CA008748"],"award-info":[{"award-number":["P30 CA008748"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The diagnosis of cutaneous \u03b3\u03b4 T\u2010cell lymphoma (GDTCL) requires the identification of \u03b3\u03b4 chains of the T\u2010cell receptor (TCR). Our aim in this study was, by using a new monoclonal antibody (mAb) against TCR\u03b4, to evaluate TCR\u03b4 expression in formalin\u2010fixed paraffin\u2010embedded (FFPE) skin tissue from TCR\u03b3+ cutaneous T\u2010cell lymphoma (CTCL), and to assess TCR\u03b4 expression within a spectrum of other cutaneous lymphoproliferative disorders (CLPDs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Twelve cases (10 patients) with TCR\u03b3+ CTCL and 132 additional CLPD cases (127 patients) were examined, including mycosis fungoides (MF) (<jats:italic>n<\/jats:italic> = 60), cutaneous GDTCL (<jats:italic>n<\/jats:italic> = 15), subcutaneous panniculitis\u2010like T\u2010cell lymphoma (SPTCL) (<jats:italic>n<\/jats:italic> = 11), and CD30<jats:sup>+<\/jats:sup> lymphoproliferative disorder (LPD) (<jats:italic>n<\/jats:italic> = 24). Clone H\u201041 against TCR\u03b4 was used on a Leica Bond\u20103 automated stainer to label FFPE slides. H\u201041 immunostaining was graded as percentage infiltrate: high (50\u2013100%), moderate (10\u201349%), and low (0\u20139%). In TCR\u03b3+ tumours, 12 of 12 (100%) patients showed TCR\u03b4 expression comparable to TCR\u03b3 expression. No (0%) TCR\u03b3+ cases were negative for TCR\u03b4. In all CLPDs, TCR\u03b4 expression was as follows: GDTCL, 16 of 20 cases (14 of 15 patients) high, two moderate, and two low; MF, 0 of 60 cases high, nine moderate, and 51 low; CD30<jats:sup>+<\/jats:sup> LPD, one of 24 cases high, two moderate, and 21 low; and SPTCL, 0 of 11 cases (0 of 9 patients) high, two moderate, and two low. Three MF\u2010like cases and one SPTCL\u2010like case showed high expression; the remainder showed low expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>mAb H\u201041 against TCR\u03b4 matches TCR\u03b3 in immunostaining FFPE tissues from GDTCL, supporting H\u201041 as a replacement for mAb \u03b33.20. TCR\u03b4 expression in our study suggests that the true occurrence of \u03b3\u03b4+ non\u2010GDTCL CTCL\/CLPD may be lower than suggested by the recent literature.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13671","type":"journal-article","created":{"date-parts":[[2018,6,12]],"date-time":"2018-06-12T11:10:40Z","timestamp":1528801840000},"page":"653-662","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["T\u2010cell receptor\u2010\u03b4 expression and \u03b3\u03b4+ T\u2010cell infiltrates in primary cutaneous \u03b3\u03b4 T\u2010cell lymphoma and other cutaneous T\u2010cell lymphoproliferative disorders"],"prefix":"10.1111","volume":"73","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0606-9186","authenticated-orcid":false,"given":"Melissa","family":"Pulitzer","sequence":"first","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shamir","family":"Geller","sequence":"additional","affiliation":[{"name":"Department of Medicine Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denise","family":"Frosina","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison","family":"Moskowitz","sequence":"additional","affiliation":[{"name":"Department of Medicine Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Horwitz","sequence":"additional","affiliation":[{"name":"Department of Medicine Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Myskowski","sequence":"additional","affiliation":[{"name":"Department of Medicine Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meenal","family":"Kheterpal","sequence":"additional","affiliation":[{"name":"Department of Medicine Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet","family":"Dogan","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achim","family":"Jungbluth","sequence":"additional","affiliation":[{"name":"Department of Pathology Memorial Sloan Kettering Cancer Center New York NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,7,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-008-0134-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2009.169"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31826a5038"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-05-1597"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-04-087288"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-09-3502"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-01-643569"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.169.4.1277"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31822067d1"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1309\/AJCPMG37MXKYPUBE"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clml.2014.06.024"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12439"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e31824433d8"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318275d1a2"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0000000000000650"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/bcj.2013.10"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12874626"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.44.12.1012"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12873333"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/cup.12830"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjd.13364"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2016.240"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhis.13671","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.13671","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T01:43:01Z","timestamp":1695001381000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.13671"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,27]]},"references-count":22,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["10.1111\/his.13671"],"URL":"https:\/\/doi.org\/10.1111\/his.13671","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,7,27]]},"assertion":[{"value":"2018-03-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}