{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,20]],"date-time":"2025-07-20T22:54:58Z","timestamp":1753052098922,"version":"3.40.5"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,1,15]],"date-time":"2019-01-15T00:00:00Z","timestamp":1547510400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["17K08742"],"award-info":[{"award-number":["17K08742"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The process of differential diagnosis between epithelioid mesothelioma and lung adenocarcinoma has been progressing; however, there are no absolute immunohistochemical markers with which to definitively diagnose epithelioid mesothelioma. The aim of this study was to search for a novel negative marker of epithelioid mesothelioma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We immunohistochemically studied the applicability of mucin 21 (<jats:styled-content style=\"fixed-case\">MUC<\/jats:styled-content>21), which was identified in our previous study, as a novel, negative diagnostic marker for epithelioid mesothelioma. Seventy epithelioid mesotheliomas and 70 lung adenocarcinomas were investigated for the expression of <jats:styled-content style=\"fixed-case\">MUC<\/jats:styled-content>21, along with other previously reported markers, by the use of immunohistochemistry. <jats:styled-content style=\"fixed-case\">MUC<\/jats:styled-content>21 was expressed in only two of the 70 (3%) epithelioid mesotheliomas, as compared with 67 of the 70 (96%) lung adenocarcinomas. The sensitivity, specificity and accuracy of negative <jats:styled-content style=\"fixed-case\">MUC<\/jats:styled-content>21 expression for differentiating epithelioid mesothelioma from lung adenocarcinoma were 97%, 96%, and 96%, respectively; these are similar to those of carcinoembryonic antigen and claudin\u20104, and better than those of thyroid transcription factor\u20101, napsin\u2010A, and mucin 4.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">MUC<\/jats:styled-content>21 could be used as an additional, novel, negative immunohistochemical marker to differentiate mesothelioma from lung adenocarcinoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13775","type":"journal-article","created":{"date-parts":[[2018,10,17]],"date-time":"2018-10-17T13:45:07Z","timestamp":1539783907000},"page":"545-554","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Mucin 21 is a novel, negative immunohistochemical marker for epithelioid mesothelioma for its differentiation from lung adenocarcinoma"],"prefix":"10.1111","volume":"74","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1431-2649","authenticated-orcid":false,"given":"Yuichiro","family":"Kai","sequence":"first","affiliation":[{"name":"Department of Pathology Graduate School of Biomedical and Health Sciences Hiroshima University Hiroshima Japan"},{"name":"Department of Surgical Oncology Research Institute for Radiation Biology and Medicine Hiroshima University Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4986-2109","authenticated-orcid":false,"given":"Vishwa J","family":"Amatya","sequence":"additional","affiliation":[{"name":"Department of Pathology Graduate School of Biomedical and Health Sciences Hiroshima University Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2474-5969","authenticated-orcid":false,"given":"Kei","family":"Kushitani","sequence":"additional","affiliation":[{"name":"Department of Pathology Graduate School of Biomedical and Health Sciences Hiroshima University Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Kambara","sequence":"additional","affiliation":[{"name":"Department of Pathology Graduate School of Biomedical and Health Sciences Hiroshima University Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Department of Pathology Graduate School of Biomedical and Health Sciences Hiroshima University Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuhiro","family":"Tsutani","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology Research Institute for Radiation Biology and Medicine Hiroshima University Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihiro","family":"Miyata","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology Research Institute for Radiation Biology and Medicine Hiroshima University Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Morihito","family":"Okada","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology Research Institute for Radiation Biology and Medicine Hiroshima University Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukio","family":"Takeshima","sequence":"additional","affiliation":[{"name":"Department of Pathology Graduate School of Biomedical and Health Sciences Hiroshima University Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,1,15]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050152"},{"key":"e_1_2_8_3_1","first-page":"491","article-title":"Malignant pleural mesothelioma: an epidemiological perspective","volume":"1","author":"Robinson BM","year":"2012","journal-title":"Ann. 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