{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T17:29:48Z","timestamp":1767374988100,"version":"3.40.5"},"reference-count":62,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,7,18]],"date-time":"2019-07-18T00:00:00Z","timestamp":1563408000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Outcomes of colorectal cancer (CRC) treatment and survival have steadily improved during the past decades, accompanied by an increased risk of developing second primary tumours and metastatic tumours at unusual sites. Metastatic CRC can show mucosal colonisation, thereby mimicking a second primary tumour. This potential confusion could lead to incorrect diagnosis and consequently inadequate treatment of the patient. The aim of this study was to differentiate between metastatic CRC and a second primary (gallbladder cancer, GBC) using a combination of standard histopathology and molecular techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Ten consecutive patients with both CRC and GBC were identified in our region using the Dutch National Pathology Archive (PALGA). Two patients served as negative controls. Histology of GBC was reviewed by nine pathologists. A combination of immunohistochemistry, microsatellite analysis, genomewide DNA copy number analysis and targeted somatic mutation analysis was used to aid in differential diagnosis. In two patients, CRC and GBC were clonally related, as confirmed by somatic mutation analysis. For one case, this was confirmed by genomewide DNA copy number analysis. However, in both cases, pathologists initially considered the GBC as a second primary tumour.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Metastatic CRC displaying mucosal colonisation is often misinterpreted as a second primary tumour. A combination of traditional histopathology and molecular techniques improves this interpretation, and lowers the risk of inadequate treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13892","type":"journal-article","created":{"date-parts":[[2019,5,2]],"date-time":"2019-05-02T08:52:58Z","timestamp":1556787178000},"page":"394-404","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Colorectal metastasis to the gallbladder mimicking a primary gallbladder malignancy: histopathological and molecular characteristics"],"prefix":"10.1111","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8669-3834","authenticated-orcid":false,"given":"Tessa J J","family":"de Bitter","sequence":"first","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ragna L A","family":"van der Linden","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shannon","family":"van Vliet","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fieke","family":"Weren","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen the Netherlands"},{"name":"Department of Pathology, Amsterdam UMC Vrije Universiteit Amsterdam, Cancer Center Amsterdam Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daoud","family":"Sie","sequence":"additional","affiliation":[{"name":"Department of Pathology, Amsterdam UMC Vrije Universiteit Amsterdam, Cancer Center Amsterdam Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bauke","family":"Ylstra","sequence":"additional","affiliation":[{"name":"Department of Pathology, Amsterdam UMC Vrije Universiteit Amsterdam, Cancer Center Amsterdam Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans C","family":"van der Linden","sequence":"additional","affiliation":[{"name":"Department of Pathology Jeroen Bosch Hospital \u2019s\u2010Hertogenbosch the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1876-7261","authenticated-orcid":false,"given":"Nikki","family":"Knijn","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marjolijn J L","family":"Ligtenberg","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen the Netherlands"},{"name":"Department of Human Genetics Radboud University Medical Center Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel S","family":"van der Post","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Femke","family":"Simmer","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iris D","family":"Nagtegaal","sequence":"additional","affiliation":[{"name":"Department of Pathology Radboud University Medical Center Nijmegen the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,7,18]]},"reference":[{"unstructured":"Dutch Cancer Registry. Available at:http:\/\/www.cijfersoverkanker.nl(accessed 6 November 2018).","key":"e_1_2_7_2_1"},{"doi-asserted-by":"publisher","key":"e_1_2_7_3_1","DOI":"10.1053\/j.gastro.2016.11.022"},{"doi-asserted-by":"publisher","key":"e_1_2_7_4_1","DOI":"10.3322\/caac.21442"},{"doi-asserted-by":"publisher","key":"e_1_2_7_5_1","DOI":"10.1016\/j.canep.2014.04.004"},{"doi-asserted-by":"publisher","key":"e_1_2_7_6_1","DOI":"10.1158\/1055-9965.EPI-06-0782"},{"doi-asserted-by":"publisher","key":"e_1_2_7_7_1","DOI":"10.5858\/2008-132-931-MPOCRF"},{"doi-asserted-by":"publisher","key":"e_1_2_7_8_1","DOI":"10.1002\/path.1711600203"},{"doi-asserted-by":"publisher","key":"e_1_2_7_9_1","DOI":"10.1097\/PAS.0b013e318211b3d2"},{"doi-asserted-by":"publisher","key":"e_1_2_7_10_1","DOI":"10.1097\/00000478-199302000-00008"},{"doi-asserted-by":"publisher","key":"e_1_2_7_11_1","DOI":"10.1378\/chest.52.2.147"},{"doi-asserted-by":"publisher","key":"e_1_2_7_12_1","DOI":"10.1097\/00000478-199709000-00007"},{"doi-asserted-by":"publisher","key":"e_1_2_7_13_1","DOI":"10.1097\/PAI.0b013e3181a75e6d"},{"doi-asserted-by":"publisher","key":"e_1_2_7_14_1","DOI":"10.1002\/ijc.31051"},{"key":"e_1_2_7_15_1","first-page":"19","article-title":"Pathology databanking and biobanking in the Netherlands, a central role for PALGA, the nationwide histopathology and cytopathology data network and archive","volume":"29","author":"Casparie M","year":"2007","journal-title":"Cell. Oncol."},{"key":"e_1_2_7_16_1","first-page":"266","volume-title":"WHO classification of tumours of the digestive system","author":"Albores\u2010Saavedra J","year":"2010"},{"key":"e_1_2_7_17_1","first-page":"309","volume-title":"TNM classification of malignant tumours","author":"Sobin LH","year":"2010"},{"doi-asserted-by":"publisher","key":"e_1_2_7_18_1","DOI":"10.1038\/sj.bjc.6604867"},{"doi-asserted-by":"publisher","key":"e_1_2_7_19_1","DOI":"10.1016\/j.jmoldx.2016.06.010"},{"doi-asserted-by":"publisher","key":"e_1_2_7_20_1","DOI":"10.1093\/bioinformatics\/btu353"},{"doi-asserted-by":"publisher","key":"e_1_2_7_21_1","DOI":"10.1101\/gr.175141.114"},{"doi-asserted-by":"publisher","key":"e_1_2_7_22_1","DOI":"10.1093\/biostatistics\/kxh008"},{"doi-asserted-by":"publisher","key":"e_1_2_7_23_1","DOI":"10.1214\/15-AOAS836"},{"doi-asserted-by":"publisher","key":"e_1_2_7_24_1","DOI":"10.1002\/sim.3866"},{"doi-asserted-by":"publisher","key":"e_1_2_7_25_1","DOI":"10.1002\/1097-0142(1950)3:1<74::AID-CNCR2820030111>3.0.CO;2-7"},{"doi-asserted-by":"publisher","key":"e_1_2_7_26_1","DOI":"10.1007\/s00261-011-9696-y"},{"doi-asserted-by":"publisher","key":"e_1_2_7_27_1","DOI":"10.1007\/s00280-009-1167-6"},{"doi-asserted-by":"publisher","key":"e_1_2_7_28_1","DOI":"10.1016\/0735-6757(94)90177-5"},{"doi-asserted-by":"publisher","key":"e_1_2_7_29_1","DOI":"10.1097\/SMJ.0b013e318188703c"},{"key":"e_1_2_7_30_1","first-page":"5308585","article-title":"Metastatic cecal adenocarcinoma to the gallbladder presenting with acute cholecystitis","volume":"2018","author":"Bukhari N","year":"2018","journal-title":"Case Rep. Oncol. Med."},{"doi-asserted-by":"publisher","key":"e_1_2_7_31_1","DOI":"10.1186\/s12957-016-0843-3"},{"doi-asserted-by":"publisher","key":"e_1_2_7_32_1","DOI":"10.1007\/s10238-015-0353-6"},{"doi-asserted-by":"publisher","key":"e_1_2_7_33_1","DOI":"10.1038\/nm.3336"},{"doi-asserted-by":"publisher","key":"e_1_2_7_34_1","DOI":"10.1016\/j.cell.2009.11.007"},{"key":"e_1_2_7_35_1","first-page":"7144","article-title":"Conversion of epithelial\u2010to\u2010mesenchymal transition to mesenchymal\u2010to\u2010epithelial transition is mediated by oxygen concentration in pancreatic cancer cells","volume":"15","author":"Chen S","year":"2018","journal-title":"Oncol. Lett."},{"doi-asserted-by":"publisher","key":"e_1_2_7_36_1","DOI":"10.1158\/2159-8290.CD-15-0893"},{"doi-asserted-by":"publisher","key":"e_1_2_7_37_1","DOI":"10.1016\/j.bbrc.2012.09.059"},{"doi-asserted-by":"publisher","key":"e_1_2_7_38_1","DOI":"10.1016\/j.prp.2010.12.005"},{"doi-asserted-by":"publisher","key":"e_1_2_7_39_1","DOI":"10.5858\/2000-124-1196-EOCAIC"},{"doi-asserted-by":"publisher","key":"e_1_2_7_40_1","DOI":"10.1038\/modpathol.3800407"},{"key":"e_1_2_7_41_1","doi-asserted-by":"crossref","first-page":"1100","DOI":"10.5858\/2005-129-1100-CIAUMO","article-title":"CDX2 is a useful marker of intestinal\u2010type differentiation: a tissue microarray\u2010based study of 629 tumors from various sites","volume":"129","author":"Lott LB","year":"2005","journal-title":"Arch. Pathol. Lab. Med."},{"doi-asserted-by":"publisher","key":"e_1_2_7_42_1","DOI":"10.1186\/1746-1596-7-9"},{"doi-asserted-by":"publisher","key":"e_1_2_7_43_1","DOI":"10.1007\/s00432-004-0658-4"},{"doi-asserted-by":"publisher","key":"e_1_2_7_44_1","DOI":"10.1007\/s12253-010-9346-7"},{"doi-asserted-by":"publisher","key":"e_1_2_7_45_1","DOI":"10.1016\/j.humpath.2006.06.010"},{"doi-asserted-by":"publisher","key":"e_1_2_7_46_1","DOI":"10.1016\/j.humpath.2009.06.008"},{"doi-asserted-by":"publisher","key":"e_1_2_7_47_1","DOI":"10.1007\/s12029-018-0162-8"},{"doi-asserted-by":"publisher","key":"e_1_2_7_48_1","DOI":"10.1016\/j.ijsu.2011.07.001"},{"doi-asserted-by":"publisher","key":"e_1_2_7_49_1","DOI":"10.1002\/1097-0142(19930915)72:6<1878::AID-CNCR2820720615>3.0.CO;2-2"},{"doi-asserted-by":"publisher","key":"e_1_2_7_50_1","DOI":"10.1590\/S1516-31802008000400004"},{"doi-asserted-by":"publisher","key":"e_1_2_7_51_1","DOI":"10.1016\/j.humpath.2004.08.011"},{"doi-asserted-by":"publisher","key":"e_1_2_7_52_1","DOI":"10.1097\/00019606-200309000-00006"},{"doi-asserted-by":"publisher","key":"e_1_2_7_53_1","DOI":"10.1016\/j.ctrv.2008.11.009"},{"doi-asserted-by":"publisher","key":"e_1_2_7_54_1","DOI":"10.1056\/NEJMoa0908721"},{"doi-asserted-by":"publisher","key":"e_1_2_7_55_1","DOI":"10.1093\/annonc\/mdw324"},{"doi-asserted-by":"publisher","key":"e_1_2_7_56_1","DOI":"10.1093\/annonc\/mdu260"},{"doi-asserted-by":"publisher","key":"e_1_2_7_57_1","DOI":"10.1016\/0959-8049(95)00323-B"},{"doi-asserted-by":"publisher","key":"e_1_2_7_58_1","DOI":"10.1002\/cncr.21621"},{"doi-asserted-by":"publisher","key":"e_1_2_7_59_1","DOI":"10.1517\/13543780903136724"},{"key":"e_1_2_7_60_1","first-page":"3055","article-title":"Gemcitabine and protracted 5\u2010fluorouracil infusion as third\u2010line chemotherapy in refractory colorectal cancer patients","volume":"28","author":"Bitossi R","year":"2008","journal-title":"Anticancer Res."},{"doi-asserted-by":"publisher","key":"e_1_2_7_61_1","DOI":"10.1155\/2004\/159347"},{"doi-asserted-by":"publisher","key":"e_1_2_7_62_1","DOI":"10.1038\/nature19057"},{"doi-asserted-by":"publisher","key":"e_1_2_7_63_1","DOI":"10.1093\/bioinformatics\/btr267"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.13892","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/his.13892","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.13892","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,9]],"date-time":"2023-09-09T23:43:09Z","timestamp":1694302989000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.13892"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,7,18]]},"references-count":62,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,9]]}},"alternative-id":["10.1111\/his.13892"],"URL":"https:\/\/doi.org\/10.1111\/his.13892","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"type":"print","value":"0309-0167"},{"type":"electronic","value":"1365-2559"}],"subject":[],"published":{"date-parts":[[2019,7,18]]},"assertion":[{"value":"2019-01-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-07-18","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}