{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T09:23:19Z","timestamp":1790414599923,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,10,2]],"date-time":"2019-10-02T00:00:00Z","timestamp":1569974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["P30CA008748"],"award-info":[{"award-number":["P30CA008748"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Salivary duct carcinoma (SDC) is an aggressive salivary malignancy that results in high mortality rates and is often resistant to chemotherapy. Anti\u2010programmed death\u20101 (PD\u20101)\/programmed death ligand\u20101 (PD\u2010L1) checkpoint inhibitors have led to dramatic improvements in patients with various cancers. Other immunotherapeutic approaches, e.g. cancer vaccines, have shown promising results. Cancer testis antigens, e.g. preferentially expressed antigen in melanoma (PRAME), are regarded as promising vaccine targets because of their tumour\u2010specific expression pattern.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We analysed the immunoexpression of PD\u2010L1, PD\u20101, major histocompatibility complex class I (MHC I) and PRAME in 53 SDCs. The immunoexpression levels of PD\u2010L1 in tumour cells (TCs) and immune cells (ICs), PD\u20101 in ICs, PRAME in TCs and MHC I in TCs were analysed, and were correlated with outcome. PRAME expression was seen in 83% of SDCs. No PRAME staining was present in normal salivary gland tissue. With the three established diagnostic algorithms proposed for head and neck squamous cell carcinoma, the criteria being a combined positive score of \u22651, TC% \u22651%, and TC% \u226525%, 35 (66%), 17 (32%) and three cases (6%), respectively, were deemed to be positive for PD\u2010L1. PD\u20101\u2010positive ICs were seen in 35 (66%) cases. MHC I down\u2010regulation was seen in 82% of SDCs. There was a significant correlation among PD\u2010L1 expression in ICs, PD\u20101 expression in ICs, and PRAME expression in TCs. PD\u2010L1 expression in TCs and lack of PD\u20101 expression in ICs were associated with decreased disease\u2010specific survival in SDC patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Alterations of the tumour immune microenvironment are common in SDCs, including expression of PD\u20101\/PD\u2010L1 and PRAME, which opens the way to potential novel immune therapies, such as cancer vaccination and PD\u20101\/PD\u2010L1 blockade, in these tumours.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13944","type":"journal-article","created":{"date-parts":[[2019,6,25]],"date-time":"2019-06-25T21:41:45Z","timestamp":1561498905000},"page":"672-682","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["The immune microenvironment and expression of PD\u2010L1, PD\u20101, PRAME and MHC I in salivary duct 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