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A novel microbiopsy forceps used during endoscopic ultrasound examinations offers new opportunities for histological examination of tissue from pancreatic cysts as well as next\u2010generation sequencing. The aim of this study was to analyse the results of next\u2010generation sequencing of microbiopsies from pancreatic cysts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Microbiopsies from 27 patients were obtained, 23 of which were subjected to next\u2010generation sequencing. Sixteen intraductal papillary mucinous neoplasms harboured mutations in genes regulating cell cycle and repair, and three were without mutations. Most frequent mutations were found in the <jats:italic>KRAS<\/jats:italic> and <jats:italic>GNAS<\/jats:italic> genes, and these were often concomitant. Three serous cystic neoplasms were without mutations, while with regard to histology, a non\u2010diagnostic microbiopsy harboured a <jats:italic>KRAS<\/jats:italic> and a <jats:italic>TP53<\/jats:italic> mutation and was deemed malignant after clinical follow\u2010up. Three patients underwent surgery, and the point mutations detected in the microbiopsies were confirmed in the resected specimens. We identified one resected sample with an additional <jats:italic>GNAS<\/jats:italic> mutation which was not identified in the microbiopsy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Next\u2010generation sequencing of microbiopsies may have the potential to improve diagnostic decision\u2010making.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.13949","type":"journal-article","created":{"date-parts":[[2019,7,6]],"date-time":"2019-07-06T15:09:32Z","timestamp":1562425772000},"page":"767-771","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Next\u2010generation sequencing of endoscopic ultrasound guided microbiopsies from pancreatic cystic neoplasms"],"prefix":"10.1111","volume":"75","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0128-1145","authenticated-orcid":false,"given":"Charlotte","family":"Vestrup Rift","sequence":"first","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital Rigshospitalet Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linea C","family":"Melchior","sequence":"additional","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital Rigshospitalet Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bojan","family":"Kovacevic","sequence":"additional","affiliation":[{"name":"Gastro Unit Copenhagen University Hospital Herlev and Gentofte Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anders","family":"Toxv\u00e6rd","sequence":"additional","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital Herlev and Gentofte Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9386-9167","authenticated-orcid":false,"given":"Pia","family":"Klausen","sequence":"additional","affiliation":[{"name":"Gastro Unit Copenhagen University Hospital Herlev and Gentofte Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John G","family":"Karstensen","sequence":"additional","affiliation":[{"name":"Gastro Unit Copenhagen University Hospital Hvidovre Hvidovre Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evangelos","family":"Kalaitzakis","sequence":"additional","affiliation":[{"name":"Gastro Unit Copenhagen University Hospital Herlev and Gentofte Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Storkholm","sequence":"additional","affiliation":[{"name":"Department of Surgery Copenhagen University Hospital Rigshospitalet Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carsten","family":"Paln\u00e6s Hansen","sequence":"additional","affiliation":[{"name":"Department of Surgery Copenhagen University Hospital Rigshospitalet Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Vilmann","sequence":"additional","affiliation":[{"name":"Gastro Unit Copenhagen University Hospital Herlev and Gentofte Herlev Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane","family":"Preuss Hasselby","sequence":"additional","affiliation":[{"name":"Department of Pathology Copenhagen University Hospital Rigshospitalet Copenhagen Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2019,9,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-313127"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra031623"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/a-0625-6440"},{"key":"e_1_2_7_5_1","first-page":"932","article-title":"Molecular biomarkers have the potential to improve the diagnostic work\u2010up of pancreatic cystic lesions","volume":"52","author":"Plougmann JI","year":"2017","journal-title":"Scand. J. Gastroenterol."},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.5154"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20084"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40064-016-2847-4"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4344"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2012.11.016"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-005-0039-7"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jamcollsurg.2014.11.029"},{"key":"e_1_2_7_13_1","first-page":"109","article-title":"Expression of von Hippel\u2010Lindau gene product (pVHL) and S100P in cystic neoplasms of the pancreas \u2013 with an implication for their roles in tumorigenesis","volume":"42","author":"Liu H","year":"2012","journal-title":"Ann. Clin. Lab. 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