{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T16:02:17Z","timestamp":1786723337654,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,5,17]],"date-time":"2020-05-17T00:00:00Z","timestamp":1589673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Rearrangement of the platelet\u2010derived growth factor receptor B (<jats:italic>PDGFRB<\/jats:italic>) gene defines a unique group of myeloid\/lymphoid neoplasms with frequent eosinophilia and high sensitivity to tyrosine kinase inhibitors. This genetic abnormality is also rarely reported in Philadelphia\u2010like B\u2010cell acute lymphoblastic leukaemia\/lymphoma (B\u2010ALL). <jats:italic>PDGFRB<\/jats:italic> rearrangement was initially thought to only occur in cases with 5q31\u201333 rearrangement as determined with conventional cytogenetics; however, there are reported cases with cryptic rearrangements. We aim to develop a broader strategy for screening of <jats:italic>PDGFRB<\/jats:italic> rearrangements of patients with myeloid\/lymphoid neoplasms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We performed fluorescence <jats:italic>in\u2010situ<\/jats:italic> hybridisation (FISH) for <jats:italic>PDGFRB<\/jats:italic> rearrangement in 197 patients, including 70 with B\u2010ALL, 10 with myeloid neoplasms with 5q31\u201333 rearrangements, and 117 with eosinophilia (\u22650.5\u00a0\u00d7\u00a010<jats:sup>9<\/jats:sup>\/l in peripheral blood or \u22655% in bone marrow), and identified <jats:italic>PDGFRB<\/jats:italic> rearrangement in four of 197 (2.0%) cases. In an attempt to identify clinicopathological and genetic features that may have a stronger association with <jats:italic>PDGFRB<\/jats:italic> rearrangement, we analysed 13 patients with confirmed <jats:italic>PDGFRB<\/jats:italic> rearrangements, including 10 with myeloid neoplasms and three with B\u2010ALL. Among the 10 patients with myeloid neoplasms, eosinophilia was present in eight, monocytosis in two, 5q31\u201333 rearrangement in seven, and abnormal bone marrow morphology in all. All patients with myeloid neoplasms showed an excellent response to imatinib, including a patient in blast crisis. The three B\u2010ALL patients presented <jats:italic>de\u00a0novo<\/jats:italic>, showed no eosinophilia, had a complex karyotype including 5q31\u201333 rearrangement, and had clinically aggressive courses with ultimate patient demise.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings suggest that a higher yield for the identification of <jats:italic>PDGFRB<\/jats:italic> rearrangement may result from an index of suspicion in patients with eosinophilia, monocytosis, bone marrow features of a myeloid neoplasm, and 5q31\u201333 rearrangement, and patients with Philadelphia\u2010like B\u2010ALL.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14097","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T15:35:37Z","timestamp":1582299337000},"page":"1042-1054","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Systematic use of fluorescence <i>in\u2010situ<\/i> hybridisation and clinicopathological features in the screening of <i>PDGFRB<\/i> rearrangements of patients with myeloid\/lymphoid neoplasms"],"prefix":"10.1111","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1780-5959","authenticated-orcid":false,"given":"Hong","family":"Fang","sequence":"first","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guilin","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8980-3202","authenticated-orcid":false,"given":"Sanam","family":"Loghavi","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Greipp","sequence":"additional","affiliation":[{"name":"Division of Laboratory Genetics Mayo Clinic Rochester MN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6821-4556","authenticated-orcid":false,"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematopathology The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srdan","family":"Verstovsek","sequence":"additional","affiliation":[{"name":"Department of Leukemia The University of Texas MD Anderson Cancer Center Houston TX USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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