{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T16:39:48Z","timestamp":1770914388571,"version":"3.50.1"},"reference-count":15,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,5,4]],"date-time":"2020-05-04T00:00:00Z","timestamp":1588550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The Cancer Genome Atlas (TCGA) provides an integrated resource for investigating the genetic, phenotypical and clinical characteristics of cancer. In this study, we aimed to define distinct subsets of clear cell renal cell carcinoma (ccRCC) through differential expression and principal component analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We used DESeq2 to examine the expression profiles of 472 cases in TCGA. After a process of segregation and regrouping, we compared the mutation and copy number variation landscapes to discern two major clusters: cluster 1, composed mainly of classic ccRCC, and cluster 2, which was associated with gains at chromosomes 7 and 12. Gene set enrichment analysis disclosed that cluster 2 tumours were enriched in genes involving epithelial\u2013mesenchymal transition. Histologically, cluster 2 tumours frequently exhibited cell elongation or spindling. Patients with cluster 2 tumours or tumours harbouring chromosomes 7 or 12 gains had a significantly greater cumulative incidence of mortality. We then employed fluorescence<jats:italic>in\u2010situ<\/jats:italic>hybridisation with probes against chromosomes 7 and 12 in a cohort of 119 cases of ccRCC from our institute for validation. Chromosomes 7 and 12 gains were associated with lower survival rates in both univariate and multivariate analyses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study demonstrates that genetic data obtained through appropriate molecular methodologies can be a useful adjunct to help predict prognosis. It also provides an example of exploring TCGA to extract meaningful information that can eventually contribute to precision medicine.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14104","type":"journal-article","created":{"date-parts":[[2020,3,14]],"date-time":"2020-03-14T07:05:11Z","timestamp":1584169511000},"page":"950-958","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Differential expression analysis of clear cell renal cell carcinomas in The Cancer Genome Atlas distinguishes an aggressive subset enriched with chromosomes 7 and 12 gains"],"prefix":"10.1111","volume":"76","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9990-8972","authenticated-orcid":false,"given":"Chin\u2010Chen","family":"Pan","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Taipei Veterans General Hospital Taipei"},{"name":"School of Medicine National Yang\u2010Ming University Taipei"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Chen","family":"Yeh","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Taipei Veterans General Hospital Taipei"},{"name":"School of Medicine National Yang\u2010Ming University Taipei"},{"name":"Institute of Biomedical Informatics National Yang\u2010Ming University Taipei"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu\u2010Chao","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Biomedical Informatics National Yang\u2010Ming University Taipei"},{"name":"Preventive Medicine Research Center National Yang\u2010Ming University Taipei"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yen\u2010Hwa","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Urology Taipei Veterans General Hospital Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,5,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12222"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2699"},{"key":"e_1_2_7_4_1","first-page":"18","volume-title":"WHO classification of tumours of the urinary system and male genital organs","author":"Moch H","year":"2019"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.03.075"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw325"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.13284"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000446"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1177\/1947601909359929"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2011.10.007"},{"key":"e_1_2_7_13_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1200\/PO.17.00011","article-title":"OncoKB: a precision oncology knowledge base","author":"Chakravarty D","year":"2017","journal-title":"JCO. 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