{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:35:41Z","timestamp":1791282941893,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,7,4]],"date-time":"2020-07-04T00:00:00Z","timestamp":1593820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Despite their low individual metastatic potential, thin melanomas (\u22641\u00a0mm Breslow thickness) contribute significantly to melanoma mortality overall. Therefore, identification of prognostic biomarkers is particularly important in this subgroup of melanoma. Prompted by preclinical results, we investigated cyclin D1 protein and Ki\u201067 expression in <jats:italic>in\u2010situ<\/jats:italic>, metastatic and non\u2010metastatic thin melanomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Immunohistochemistry was performed on 112\u00a0melanoma specimens, comprising 22\u00a0<jats:italic>in situ<\/jats:italic>, 48\u00a0non\u2010metastatic and 42\u00a0metastatic thin melanomas. Overall, epidermal and dermal cyclin D1 and Ki\u201067 expression were semiquantitatively evaluated by three independent investigators and compared between groups. Epidermal Ki\u201067 expression did not differ statistically in <jats:italic>in\u2010situ<\/jats:italic> and invasive melanoma (<jats:italic>P\u00a0=\u00a0<\/jats:italic>0.7). Epidermal cyclin D1 expression was significantly higher in thin invasive than in <jats:italic>in\u2010situ<\/jats:italic> melanoma (<jats:italic>P\u00a0=\u00a0<\/jats:italic>0.003). No difference was found in cyclin D1 expression between metastatic and non\u2010metastatic invasive tumours. Metastatic and non\u2010metastatic thin melanomas did not show significant differences in epidermal expression of Ki\u201067 and cyclin D1 (<jats:italic>P\u00a0=\u00a0<\/jats:italic>0.148 and <jats:italic>P\u00a0=\u00a0<\/jats:italic>0.611, respectively). In contrast, strong dermal expression of Ki\u201067 was more frequent in metastatic than non\u2010metastatic samples (28.6 versus 8.3%, respectively, <jats:italic>P\u00a0=\u00a0<\/jats:italic>0.001). The prognostic value of dermal Ki\u201067 expression was confirmed by multivariate analysis (<jats:italic>P\u00a0=\u00a0<\/jats:italic>0.047).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We found an increased expression of cyclin D1 in invasive thin melanomas compared to <jats:italic>in\u2010situ<\/jats:italic> melanomas, which supports a potential role of this protein in early invasion in melanoma, as suggested by preclinical findings. Moreover, our results confirm that high dermal Ki\u201067 expression is associated with an increased risk of development of metastasis in thin melanoma and could possibly serve as a prognostic biomarker in clinical practice, especially if combined with additional methods.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14139","type":"journal-article","created":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T20:06:26Z","timestamp":1588795586000},"page":"460-470","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The role of cyclin D1 and Ki\u201067 in the development and prognostication of thin melanoma"],"prefix":"10.1111","volume":"77","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1686-9451","authenticated-orcid":false,"given":"Corina","family":"Kaufmann","sequence":"first","affiliation":[{"name":"Department of Dermatology University Hospital Zurich Zurich Switzerland"},{"name":"University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Werner","family":"Kempf","sequence":"additional","affiliation":[{"name":"Kempf and Pfaltz Histological Diagnostics Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Mangana","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phil","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Emberger","sequence":"additional","affiliation":[{"name":"Private Pathological Laboratory Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Lang","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Allergology Paracelsus Medical University Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas K","family":"Kaiser","sequence":"additional","affiliation":[{"name":"Department of Clinical Psychology Christian\u2010Doppler\u2010Klinik Paracelsus Medical University Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn","family":"Lattmann","sequence":"additional","affiliation":[{"name":"University of Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell","family":"Levesque","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Dummer","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Zurich Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Koelblinger","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Hospital Zurich Zurich Switzerland"},{"name":"Department of Dermatology and Allergology Paracelsus Medical University Salzburg Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,7,4]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"599.e1\u2013e12","article-title":"Screening, early detection, education, and trends for melanoma: current status (2007\u20132013) and future directions: Part I. 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