{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:51:29Z","timestamp":1787014289373,"version":"build-2736575974"},"reference-count":51,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,10,27]],"date-time":"2020-10-27T00:00:00Z","timestamp":1603756800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Differentiated vulvar intraepithelial neoplasia (dVIN), the precursor lesion to human papillomavirus\u2010independent vulvar squamous cell carcinoma (VSCC), can be difficult to distinguish from vulvar inflammatory dermatoses. Our goal was to determine if p53 could be a useful biomarker for dVIN, by characterizing p53 percentage, intensity and patterns of staining in dVIN and its histological mimics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>We studied p53 immunohistochemical staining patterns in 16 dVIN cases and 46 vulvar non\u2010neoplastic squamous lesions [12 lichen sclerosus (LS); seven lichen simplex chronicus; three lichen planus (LP); six psoriasis; 13 spongiotic dermatitis (SPO); and five candidiasis]. dVIN cases were adjacent to a p16\u2010negative invasive VSCC in resection specimens. All dVIN cases showed null\u2010type or moderate to strong uniform p53 staining in &gt;70% of basal cells, with moderate to strong continuous parabasal staining extending to two\u2010thirds of the epidermis. This was in contrast to weak or weak to moderate patchy p53 staining in the majority of other lesions. Moderate to strong and increased basal p53 staining (\u226570%) was also observed in a subset of LS cases (5\/12, 42%), LP cases (1\/3, 33%), and SPO cases (36%, 4\/11); however, in all categories, this was limited to the basal layer, and any staining in the parabasal layers was patchy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Strong and uniform p53 staining of basal cells, extending into the parabasal layers, and a complete absence of staining (null type) is useful in distinguishing dVIN from other mimics in the vulva. p53 staining of lesser intensity or quantity, particularly basal overexpression only, overlaps with that in vulvar inflammatory lesions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.14238","type":"journal-article","created":{"date-parts":[[2020,8,16]],"date-time":"2020-08-16T12:25:00Z","timestamp":1597580700000},"page":"424-433","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Comparison of p53 immunohistochemical staining in differentiated vulvar intraepithelial neoplasia (dVIN) with that in inflammatory dermatoses and benign squamous lesions in the vulva"],"prefix":"10.1111","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5382-8774","authenticated-orcid":false,"given":"Y Ariel","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9794-8222","authenticated-orcid":false,"given":"Jennifer X","family":"Ji","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver BC Canada"},{"name":"Molecular Oncology British Columbia Cancer Research Centre Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noorah","family":"Almadani","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard I","family":"Crawford","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver BC Canada"},{"name":"Department of Dermatology and Skin Science University of British Columbia Vancouver BC Canada"},{"name":"Division of Anatomical Pathology Vancouver General Hospital Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7889-8250","authenticated-orcid":false,"given":"C Blake","family":"Gilks","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver BC Canada"},{"name":"Division of Anatomical Pathology Vancouver General Hospital Vancouver BC Canada"},{"name":"Genetic Pathology Evaluation Center (GPEC) Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Kinloch","sequence":"additional","affiliation":[{"name":"Department of Pathology Saskatoon City Hospital Saskatoon SK Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3692-8057","authenticated-orcid":false,"given":"Lien","family":"Hoang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of British Columbia Vancouver BC Canada"},{"name":"Division of Anatomical Pathology Vancouver General Hospital Vancouver BC Canada"},{"name":"Genetic Pathology Evaluation Center (GPEC) Vancouver BC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,10,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.AOG.0000210268.57527.a1"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathol.2016.02.015"},{"key":"e_1_2_9_4_1","first-page":"807","article-title":"Squamous vulvar intraepithelial neoplasia: 2004 modified terminology, ISSVD Vulvar Oncology Subcommittee","volume":"50","author":"Sideri M","year":"2005","journal-title":"J. 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