{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T03:24:02Z","timestamp":1787887442043,"version":"build-2784847793"},"reference-count":20,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,11,28]],"date-time":"2020-11-28T00:00:00Z","timestamp":1606521600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Histopathological examination of sentinel lymph nodes in penile cancer has varied during the first 10\u00a0years after introduction of the dynamic sentinel node (SLN) procedure in Denmark, and guidelines have been sparse. The aim of this study was to investigate the impact of rigorous step\u2010sectioning of sentinel lymph nodes in penile cancer and improve guidelines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Seventy\u2010two penile squamous cell carcinoma patients undergoing SLN procedure at a single institution in 2000\u20132010 were included. The archived lymph node tissues already subjected to a standard pathological examination were retrieved and the initially negative lymph nodes were subjected to an extended step\u2010sectioning procedure. The results were compared to clinical patient outcome from a national database and subsequent pathology reports. The original histopathological examination had detected 26\u00a0SLN with metastasis, 21\u00a0macro metastases and five micro metastases. The additional step\u2010sectioning procedure of this study generated 4606 slides; seven SLN metastases, two macro metastases and five micro metastases, were detected. One of the macro metastases originated from a patient in which a clinical relapse had occurred in the same groin. None of the other metastases detected in this study showed ipsilateral relapse during follow\u2010up.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results underline the value of our current practice of step\u2010sectioning sentinel lymph nodes in penile cancer and the need for histopathological routines and guidelines. The Danish national guidelines on histopathological handling of sentinel lymph nodes have been adapted to detect any potential clinically relevant metastases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14261","type":"journal-article","created":{"date-parts":[[2020,9,26]],"date-time":"2020-09-26T19:04:17Z","timestamp":1601147057000},"page":"627-633","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Lessons learned from histological step\u2010sectioning of sentinel lymph nodes in penile cancer"],"prefix":"10.1111","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9391-1764","authenticated-orcid":false,"given":"Johanna","family":"Elversang","sequence":"first","affiliation":[{"name":"Department of Pathology, Rigshospitalet University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgitte","family":"Gr\u00f8nk\u00e6r Toft","sequence":"additional","affiliation":[{"name":"Department of Pathology, Rigshospitalet University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kim","family":"Predbj\u00f8rn Krarup","sequence":"additional","affiliation":[{"name":"Department of Urology, Rigshopitalet University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakob K","family":"Jakobsen","sequence":"additional","affiliation":[{"name":"Department of Urology Aarhus University Hospital Aarhus Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,28]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2006.10.060"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.urology.2010.04.008"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11670-012-0090-2"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197702)39:2<456::AID-CNCR2820390214>3.0.CO;2-I"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2014.10.017"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2008.20.6870"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)65169-5"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.13243"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2012.11437.x"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.13127"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1008108"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.surge.2016.07.002"},{"key":"e_1_2_9_14_1","unstructured":"BriarlyJD GospodarowiczMK WittekindCeds.TNM classification of malignant tumours 8th edn. 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