{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T16:23:59Z","timestamp":1791563039701,"version":"4.3.4"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T00:00:00Z","timestamp":1608854400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100012543","name":"Shanghai Science and Technology Development Foundation","doi-asserted-by":"publisher","award":["19MC1911000"],"award-info":[{"award-number":["19MC1911000"]}],"id":[{"id":"10.13039\/100012543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Very limited data are available concerning the clinicopathological and molecular features of early subungual melanoma (SM), especially with regard to the Asian population. The aim of this study was to investigate the clinical, histological, immunohistochemical and chromosomal features of early SM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Fifty\u2010two <jats:italic>in\u2010situ<\/jats:italic> and 13 thin (Breslow thickness \u22641.0\u00a0mm) SM cases were retrospectively reviewed. All patients presented with longitudinal melanonychia involving a single digit, and the thumb was the most affected digit (35 of 65, 53.8%). Microscopically, most cases showed small to medium nuclear enlargement (58 of 65) and mild to moderate nuclear atypia (57 of 65). Hyperchromatism and irregular contours of nuclei were persistent features in all cases. The variation of melanocyte count (the number of melanocytes per mm dermal\u2010epithelial junction) ranged from 31 to 255. Intra\u2010epithelial mitoses were identified in 34 cases (52.3%). Statistically, features of <jats:italic>in\u2010situ<\/jats:italic> lesions including higher melanocyte count (&gt;70), presence of multinucleated melanocytes, inflammatory infiltrate and cutaneous adnexal extension, were associated with early invasion. Melan\u2010A, human melanoma B (HMB)45, mouse monoclonal melanoma antibody (PNL2) and SOX10 antibodies (&gt;95.0%) showed superior diagnostic sensitivity to S\u2010100 protein (83.1%). Fluorescence <jats:italic>in\u2010situ<\/jats:italic> hybridisation (FISH) results were positive in 15 of 23 successfully analysed cases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>To the best of our knowledge, this is the largest single\u2010institution study of early SM in an Asian population, and the largest cohort tested by FISH. Early SM mainly showed small to medium nuclear enlargement and mild to moderate nuclear atypia. High melanocyte count, hyperchromatism and irregular contours of nuclei and intra\u2010epithelial mitoses are crucial diagnostic parameters. Immunohistochemistry, especially SOX10 staining, and FISH analysis are valuable in the diagnosis of SM.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14279","type":"journal-article","created":{"date-parts":[[2020,10,16]],"date-time":"2020-10-16T21:15:52Z","timestamp":1602882952000},"page":"717-726","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Clinicopathological, immunohistochemical and fluorescence <i>in\u2010situ<\/i> hybridisation features of early subungual melanoma: an analysis of 65 cases"],"prefix":"10.1111","volume":"78","author":[{"given":"Min","family":"Ren","sequence":"first","affiliation":[{"name":"Department of Pathology Fudan University Shanghai Cancer Center Shanghai China"},{"name":"Department of Oncology Shanghai Medical College Fudan University Shanghai 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