{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T03:54:41Z","timestamp":1787630081180,"version":"build-2736575974"},"reference-count":15,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,3,16]],"date-time":"2021-03-16T00:00:00Z","timestamp":1615852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["KL2TR003143"],"award-info":[{"award-number":["KL2TR003143"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>Mucin 4 (MUC4) is a transmembrane glycoprotein normally expressed by several human epithelial surfaces, including those of the colon, vagina, and respiratory tract. Although MUC4 overexpression is seen in various carcinomas, its expression among mesenchymal neoplasms is fairly specific to low\u2010grade fibromyxoid sarcoma and sclerosing epithelioid fibrosarcoma. Having observed unanticipated anti\u2010MUC4 immunoreactivity in rhabdomyosarcoma, we aimed to further characterise its expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>\n                      Expression of MUC4 was assessed by immunohistochemistry in a total of 97 rhabdomyosarcomas using formalin\u2010fixed paraffin\u2010embedded tissue sections. MUC4 was expressed by 21 of 26\n                      <jats:italic>PAX3\/7\u2013FOXO1<\/jats:italic>\n                      fusion\u2010positive cases, wherein immunoreactivity, varying from weak to strong, was present in 20\u2013100% of neoplastic cells. With the exception of one sclerosing rhabdomyosarcoma showing immunoreactivity in 20% of cells, MUC4 was not expressed by embryonal (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a028), sclerosing (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a020), or pleomorphic (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a023) rhabdomyosarcomas. Analysing published gene expression microarray data from a separate cohort of 33 fusion\u2010positive and 25 fusion\u2010negative rhabdomyosarcomas, we found on average a 11.4\u2010fold increased expression in fusion\u2010positive tumours (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a00.0004).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      MUC4 is expressed to a variable extent in the majority of\n                      <jats:italic>PAX3\/7\u2013FOXO1<\/jats:italic>\n                      fusion\u2010positive (alveolar) rhabdomyosarcomas, while expression in other rhabdomyosarcoma subtypes is rare.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.14321","type":"journal-article","created":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T04:49:50Z","timestamp":1608871790000},"page":"905-908","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["MUC4 is expressed in alveolar rhabdomyosarcoma"],"prefix":"10.1111","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6320-0905","authenticated-orcid":false,"given":"Erna","family":"Forg\u00f3","sequence":"first","affiliation":[{"name":"Department of Pathology Stanford University School of Medicine Stanford CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6475-8345","authenticated-orcid":false,"given":"Jason L","family":"Hornick","sequence":"additional","affiliation":[{"name":"Department of Pathology Brigham and Women's Hospital and Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2774-2704","authenticated-orcid":false,"given":"Gregory W","family":"Charville","sequence":"additional","affiliation":[{"name":"Department of Pathology Stanford University School of Medicine Stanford CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,3,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2217\/fon.09.125"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3114"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318210c268"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3182562bf8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0000000000000816"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-019-02623-8"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.13730"},{"key":"e_1_2_8_9_1","article-title":"Intracranial mesenchymal tumor with FET\u2010CREB fusion \u2013 a unifying diagnosis for the spectrum of intracranial myxoid mesenchymal tumors and angiomatoid fibrous histiocytoma\u2010like neoplasms","author":"Sloan 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