{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T04:46:48Z","timestamp":1793249208633,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,4,14]],"date-time":"2021-04-14T00:00:00Z","timestamp":1618358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2021,6]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Methylthioadenosine phosphorylase (MTAP) immunohistochemical expression is a specific marker of <jats:italic>CDKN2A<\/jats:italic> deletion in malignant mesothelioma. However, the relationship of MTAP expression with <jats:italic>MTAP<\/jats:italic> copy number remains unexplored.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Forty malignant pleural mesotheliomas were characterised by targeted next\u2010generation sequencing (29), single\u2010nucleotide polymorphism microarray (seven), or both (four). <jats:italic>MTAP<\/jats:italic> and <jats:italic>CDKN2A<\/jats:italic> copy numbers were correlated with MTAP expression. Twenty\u2010seven (68%) tumours showed <jats:italic>CDKN2A<\/jats:italic> deletion (14 heterozygous; 13 homozygous), of which 20 (74%) showed <jats:italic>MTAP<\/jats:italic> codeletion (15 heterozygous; five homozygous). No tumours showed <jats:italic>MTAP<\/jats:italic> deletion without <jats:italic>CDKN2A<\/jats:italic> codeletion. Loss of MTAP expression was seen in 16 (40%) tumours, and was 75% sensitive and 95% specific for <jats:italic>MTAP<\/jats:italic> deletion, and 59% sensitive and 100% specific for <jats:italic>CDKN2A<\/jats:italic> deletion. Nine of 40 (23%) tumours showed heterogeneous MTAP staining, and the percentage of tumour cells with MTAP loss correlated with molecular detection of <jats:italic>MTAP<\/jats:italic> deletion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>MTAP<\/jats:italic> is frequently codeleted with <jats:italic>CDKN2A<\/jats:italic> in pleural mesothelioma. However, homozygous deletion of both genes occurs in a minority of tumours (5\/40; 13%); <jats:italic>CDKN2A<\/jats:italic> deletion often co\u2010occurs with heterozygous <jats:italic>MTAP<\/jats:italic> deletion or neutral <jats:italic>MTAP<\/jats:italic> copy number; and MTAP expression correlates inconsistently with heterozygous <jats:italic>MTAP<\/jats:italic> deletion. Correspondingly, MTAP immunohistochemistry is a highly specific but only moderately sensitive assay for <jats:italic>CDKN2A<\/jats:italic> deletion.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14324","type":"journal-article","created":{"date-parts":[[2021,1,2]],"date-time":"2021-01-02T14:18:33Z","timestamp":1609597113000},"page":"1032-1042","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":48,"title":["Correlation of methylthioadenosine phosphorylase (MTAP) protein expression with <i>MTAP<\/i> and <i>CDKN2A<\/i> copy number in malignant pleural mesothelioma"],"prefix":"10.1111","volume":"78","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9733-5442","authenticated-orcid":false,"given":"David B","family":"Chapel","sequence":"first","affiliation":[{"name":"Department of Pathology Brigham and Women's Hospital and Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian M","family":"Dubuc","sequence":"additional","affiliation":[{"name":"Department of Pathology Brigham and Women's Hospital and Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6475-8345","authenticated-orcid":false,"given":"Jason L","family":"Hornick","sequence":"additional","affiliation":[{"name":"Department of Pathology Brigham and Women's Hospital and Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynette M","family":"Sholl","sequence":"additional","affiliation":[{"name":"Department of Pathology Brigham and Women's Hospital and Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,4,14]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"319","article-title":"Malignant pleural mesothelioma: incidence, etiology, diagnosis, treatment, and occupational health","volume":"110","author":"Neumann V","year":"2013","journal-title":"Dtsch. 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