{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T20:53:38Z","timestamp":1785876818195,"version":"3.56.0"},"reference-count":58,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2021,5,7]],"date-time":"2021-05-07T00:00:00Z","timestamp":1620345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Oropharyngeal squamous cell carcinomas (OPSCC) related to human papillomavirus (HPV) infection have a better prognosis than those without HPV infection. Although p16<jats:sup>INK4a<\/jats:sup> overexpression is used as a surrogate marker for HPV infection, 5\u201320% of p16\u2010positive OPSCC are described as being unrelated to HPV infection, with worse overall survival compared to OPSCC\u2010related HPV. There is therefore a risk of undertreating a proportion of OPSCC patients falsely considered to be HPV\u2010driven because of p16 positivity. <jats:italic>TP53<\/jats:italic> mutations are highly prevalent in OPSCC driven by mutagens in tobacco and alcohol. We describe herein a combined p16\/p53 algorithm to predict HPV tumour status in OPSCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>A total of 110 OPSCC were identified in the database of the pathology department and were studied using p16 and p53 immunohistochemistry. For p16\u2010positive or p16\u2010negative\/wild\u2010type patterns\u2010p53 (WT\u2010p53) cases (<jats:italic>n<\/jats:italic>\u00a0=\u00a063), DNA <jats:italic>in\u2010situ<\/jats:italic> hybridisation for high\u2010risk HPV was performed, and if negative the HPV status was controlled by HPV DNA polymerase chain reaction (PCR) (<jats:italic>n<\/jats:italic>\u00a0=\u00a019). A significant association between <jats:italic>TP53<\/jats:italic> mutation and pattern of p53 expression was found (WT\u2010p53, seven of 16, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). The p16\u2010positive\/WT\u2010p53 was significantly associated with HPV<jats:sup>+<\/jats:sup> tumour status (p16\u2010positive\/WT\u2010p53, 50 of 110, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Interestingly, a subset of p16\u2010positive OPSCC was unrelated to HPV (13.5%, eight of 59), and showed mutant\u2010type staining of p53 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The p16 protein immunopositivity in conjunction with the mutant\u2010type pattern of p53 staining helped to reclassify a subset of p16\u2010positive OPSCC as OPSCC\u2010unrelated HPV. This approach could be routinely applied by pathologists involved in the management of OPSCC, because of their potential therapeutic implications.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14350","type":"journal-article","created":{"date-parts":[[2021,2,10]],"date-time":"2021-02-10T07:04:45Z","timestamp":1612940685000},"page":"381-390","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Oropharyngeal squamous cell carcinoma: p16\/p53 immunohistochemistry as a strong predictor of HPV tumour status"],"prefix":"10.1111","volume":"79","author":[{"given":"Nazim","family":"Benzerdjeb","sequence":"first","affiliation":[{"name":"Department of Pathology Institut de Pathologie Multisite Groupement Hospitalier Sud Hospices Civils de Lyon Pierre\u2010B\u00e9nite France"},{"name":"Universit\u00e9 Lyon 1 Villeurbanne H\u00f4pital La Croix Rousse Hospices Civils de Lyon Lyon 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