{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T18:00:52Z","timestamp":1792519252031,"version":"4.5.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,9,22]],"date-time":"2021-09-22T00:00:00Z","timestamp":1632268800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772865"],"award-info":[{"award-number":["81772865"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Pleomorphic adenoma (PA) of the breast, and especially its malignant transformation, is extremely rare and represents a diagnostic pitfall. Molecular alterations in this entity have not been investigated. We aimed to examine the clinicopathological features of our breast PAs and perform molecular analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Seven cases of breast PA, including two cases of carcinoma ex PA, were analysed. <jats:italic>PLAG1<\/jats:italic> and <jats:italic>HMGA2<\/jats:italic> gene rearrangements were assayed by fluorescence <jats:italic>in\u2010situ<\/jats:italic> hybridisation (FISH) and RNA sequencing (RNA\u2010Seq), respectively. Reverse transcription\u2013polymerase chain reaction (RT\u2013PCR) and Sanger sequencing were used to verify RNA sequencing results. All seven cases of breast PA occurred in women. The histological features were similar to the analogous tumour in salivary glands, including a dual epithelial\u2013myoepithelial component and negativity of oestrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER2) by immunohistochemistry. Of the two cases with carcinoma ex PA, one demonstrated minimal invasion and one was extensively invasive. <jats:italic>PLAG1<\/jats:italic> rearrangements were identified in two cases (28.6%), but no rearrangements of <jats:italic>HMG2A<\/jats:italic> were found. A novel fusion product in PAs, <jats:italic>TRPS1\u2013PLAG1<\/jats:italic>, was identified in one case. No patients had recurrence or metastasis with a follow\u2010up period of 6\u2013158\u2009months.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Breast PA is rare, but it is an important differential diagnosis of breast pathology with the potential to develop carcinoma ex PA. We report a novel <jats:italic>TRPS1\u2013PLAG1<\/jats:italic> fusion gene in breast PA.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14461","type":"journal-article","created":{"date-parts":[[2021,7,22]],"date-time":"2021-07-22T15:54:04Z","timestamp":1626969244000},"page":"1030-1039","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Some pleomorphic adenomas of the breast share <i>PLAG1<\/i> rearrangements with the analogous tumour of the salivary glands"],"prefix":"10.1111","volume":"79","author":[{"given":"Shirong","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Pathology The Basic Medicine Science and the First Affiliated Hospital of the Air Force Military Medical University Xi\u2019an Shaan Xi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Danhui","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Pathology The Basic Medicine 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Air Force Military Medical University Xi\u2019an Shaan Xi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linni","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Pathology The Basic Medicine Science and the First Affiliated Hospital of the Air Force Military Medical University Xi\u2019an Shaan Xi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junhui","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of Pathology The Basic Medicine Science and the First Affiliated Hospital of the Air Force Military Medical University Xi\u2019an Shaan Xi Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology The Basic Medicine Science and the First Affiliated Hospital of the Air Force Military Medical University Xi\u2019an Shaan Xi Province 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