{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:13:01Z","timestamp":1747185181565,"version":"3.40.5"},"reference-count":40,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T00:00:00Z","timestamp":1648080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011539","name":"Masonic Cancer Center, University of Minnesota","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100011539","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:sec><jats:title>Background and aims<\/jats:title><jats:p>Cutaneous melanoma often metastasises <jats:italic>in primis<\/jats:italic> to sentinel lymph nodes (SLNs). Currently, there is no standardized method of characterizing the micrometastatic tumour burden in SLN biopsies for melanoma. Different criteria have been developed to evaluate SLN biopsies, yet none consider the number of cells identified. Here, we used software analysis to digitally quantify metastatic tumour burden within SLNs and correlated these data with clinicopathological and prognostic information.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We identified 246 cases of SLN biopsies, including 63 positive (26%) and183 (74%) negative for metastatic melanoma. Digital cell counting was performed within the greatest metastatic focus and the entire metastatic tumour burden within the same SLN. Increasing cell count in the largest metastatic deposit correlated with the previously described Rotterdam [Spearman's <jats:italic>r<\/jats:italic>\u2009=\u20090.91; 95% confidence interval (CI)\u2009=\u20090.84, 0.94], Starz (Spearman's <jats:italic>r<\/jats:italic>\u2009=\u20090.78; 95% CI\u2009=\u20090.68, 0.87) and Dewar criteria (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01), validating our method of using cell count to define SLN tumour burden. Additionally, increasing cell count was associated with decreased metastasis\u2010free survival (hazard ratio\u2009=\u20092.29; 95% CI\u2009=\u20091.22, 4.31).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These data support the use of computerized cell count analysis for prognostication of outcomes in patients undergoing SLN biopsy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14541","type":"journal-article","created":{"date-parts":[[2021,8,17]],"date-time":"2021-08-17T09:32:43Z","timestamp":1629192763000},"page":"954-964","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Sentinel lymph node tumour burden using digital cell count estimation predicts outcomes in melanoma"],"prefix":"10.1111","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5308-9673","authenticated-orcid":false,"given":"Emily M","family":"Hartsough","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota  Minneapolis MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Miller","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota  Minneapolis MN USA"},{"name":"Department of Dermatology University of Minnesota  Minneapolis MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"Shanley","sequence":"additional","affiliation":[{"name":"Masonic Cancer Center Biostatistics Core University of Minnesota  Minneapolis MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2082-554X","authenticated-orcid":false,"given":"Evidio","family":"Domingo\u2010Musibay","sequence":"additional","affiliation":[{"name":"Masonic Cancer Center Biostatistics Core University of Minnesota  Minneapolis MN USA"},{"name":"Department of Medicine University of Minnesota  Minneapolis MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5352-0662","authenticated-orcid":false,"given":"Alessio","family":"Giubellino","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Pathology University of Minnesota  Minneapolis MN USA"},{"name":"Masonic Cancer Center Biostatistics Core University of Minnesota  Minneapolis MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,3,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21551"},{"key":"e_1_2_8_3_1","unstructured":"SEER Cancer Stat Facts.Melanoma of the skin [internet].Bethesda MD[Accessed April 21 2021]: Available at:https:\/\/seer.cancer.gov\/statfacts\/html\/melan.html."},{"volume-title":"Survival rates for melanoma skin cancer","year":"2021","author":"American Cancer Society","key":"e_1_2_8_4_1"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.3.976"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.sla.0000181092.50141.fa"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2009.27.1627"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1310460"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1613210"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-19-1194"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0000000000000481"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2012-0550-OA"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/DAD.0b013e31829432c7"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1245\/ASO.2003.03.054"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdl176"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02574515"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2007.07937.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.143.9.892"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-009-0884-8"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/0284186X.2021.1892820"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-010-1145-6"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2004.12.177"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1245\/ASO.2004.12.920"},{"key":"e_1_2_8_24_1","first-page":"301","article-title":"Comparison of three micromorphometric pathology classifications of melanoma metastases in the sentinel node","volume":"250","author":"Ploeg IMC","year":"2009","journal-title":"Ann. Oncol."},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/SLA.0b013e3181a77eba"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3800117"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1309\/TDWJTR15TDM1TG7Q"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.15.4179"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjps.2009.07.043"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181b0bd4f"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17204-5"},{"volume-title":"AJCC cancer staging manual","year":"2018","author":"Amin MB","key":"e_1_2_8_32_1"},{"key":"e_1_2_8_33_1","first-page":"646","volume-title":"Spline functions for logistic regression modeling","author":"Devlin TF","year":"1986"},{"key":"e_1_2_8_34_1","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1007\/978-3-319-19425-7","volume-title":"Regression modeling strategies","author":"Harrell FE","year":"2015"},{"volume-title":"R: a language and environment for statistical computing [internet]","year":"2017","author":"Team RC","key":"e_1_2_8_35_1"},{"key":"e_1_2_8_36_1","unstructured":"TherneauT.A package for survival analysis in S version 2.38[internet].2015; [Accessed March 1 2021]: Available at:https:\/\/cran.r\u2010project.org\/package=survival."},{"volume-title":"RMS: regression modeling strategies. R package version 5.1\u20133.1","year":"2019","author":"Harrell FE","key":"e_1_2_8_37_1"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.24298"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.03960.x"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2018.00036"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-11-43"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.14541","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/his.14541","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.14541","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T16:28:48Z","timestamp":1692980928000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.14541"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,3,24]]},"references-count":40,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1111\/his.14541"],"URL":"https:\/\/doi.org\/10.1111\/his.14541","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"type":"print","value":"0309-0167"},{"type":"electronic","value":"1365-2559"}],"subject":[],"published":{"date-parts":[[2022,3,24]]},"assertion":[{"value":"2021-04-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-08-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}