{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T12:20:32Z","timestamp":1790252432578,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T00:00:00Z","timestamp":1651622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate Epstein\u2010Barr virus (EBV) latency types in 19 cases of EBV\u2010positive nodular lymphocyte\u2010predominant Hodgkin lymphoma (NLPHL), as such information is currently incomplete.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>Immunohistochemistry (IHC) for CD20, CD79a, PAX5, OCT2, CD30, CD15, CD3 and programmed cell death protein 1 was performed. For EBV detection, <jats:italic>in\u2010situ<\/jats:italic> hybridisation (ISH) for EBV\u2010encoded RNA (EBER) was employed combined with IHC for EBV\u2010encoded latent membrane protein (LMP)\u20101, EBV\u2010encoded nuclear antigen (EBNA)\u20102, and EBV\u2010encoded BZLF1. In 95% of the cases, neoplastic cells with features of Hodgkin and Reed\u2013Sternberg (HRS) cells were present, mostly showing expression of CD30. In all cases, the B\u2010cell phenotype was largely intact, and delineation from classic Hodgkin lymphoma (CHL) was further supported by myocyte enhancer factor 2B (MEF2B) detection. All tumour cells were EBER\u2010positive except in two cases. EBV latency type II was most frequent (89%) and type I was rare. Cases with latency type I were CD30\u2010negative. Five cases contained some BZLF1\u2010positive and\/or EBNA\u20102\u2010positive bystander lymphocytes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>As HRS morphology of neoplastic cells and CD30 expression are frequent features of EBV\u2010positive NLPHL, preservation of the B\u2010cell transcription programme, MEF2B expression combined with NLPHL\u2010typical architecture and background composition facilitate distinction from CHL. EBER ISH is the method of choice to identify these cases. The majority present with EBV latency type II, and only rare cases present with latency type I, which can be associated with missing CD30 expression. The presence of occasional bystander lymphocytes expressing BZLF1 and\/or EBNA\u20102 and the partial EBV infection of neoplastic cells in some cases could indicate that EBV is either not primarily involved or is only a transient driver in the pathogenesis of EBV\u2010positive NLPHL.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.14652","type":"journal-article","created":{"date-parts":[[2022,3,24]],"date-time":"2022-03-24T21:08:44Z","timestamp":1648156124000},"page":"1071-1080","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Epstein\u2013Barr virus infection patterns in nodular lymphocyte\u2010predominant Hodgkin lymphoma"],"prefix":"10.1111","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2134-2774","authenticated-orcid":false,"given":"Elena","family":"Gerhard\u2010Hartmann","sequence":"first","affiliation":[{"name":"Institute of Pathology University of W\u00fcrzburg"},{"name":"Comprehensive Cancer Centre Mainfranken  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Korinna","family":"J\u00f6hrens","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Hospital Carl Gustav Carus, TU Dresden  Dresden Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa\u2010Marie","family":"Schinagl","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of W\u00fcrzburg"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6203-7966","authenticated-orcid":false,"given":"Alberto","family":"Zam\u00f3","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of W\u00fcrzburg"},{"name":"Comprehensive Cancer Centre Mainfranken  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Rosenwald","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of W\u00fcrzburg"},{"name":"Comprehensive Cancer Centre Mainfranken  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ioannis","family":"Anagnostopoulos","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of W\u00fcrzburg"},{"name":"Comprehensive Cancer Centre Mainfranken  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias","family":"Rosenfeldt","sequence":"additional","affiliation":[{"name":"Institute of Pathology University of W\u00fcrzburg"},{"name":"Comprehensive Cancer Centre Mainfranken  W\u00fcrzburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,4]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)90943-J"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V80.2.484.484"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711660102"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711680405"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V81.2.462.462"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/mpo.2950240102"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.10.3922.bloodjournal86103922"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428199709039015"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19960301)77:5<949::AID-CNCR22>3.0.CO;2-2"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V87.9.3828.bloodjournal8793828"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.74.1.27"},{"key":"e_1_2_9_13_1","first-page":"366","article-title":"Childhood Hodgkin's disease in the United States: An analysis of histologic subtypes and association with Epstein\u2010Barr virus","volume":"10","author":"Andriko JA","year":"1997","journal-title":"Mod. Pathol."},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/108.6.687"},{"key":"e_1_2_9_15_1","first-page":"1889","article-title":"European task force on lymphoma project on lymphocyte predominance Hodgkin disease: Histologic and immunohistologic analysis of submitted cases reveals 2 types of Hodgkin disease with a nodular growth pattern and abundant lymphocytes","volume":"96","author":"Anagnostopoulos I","year":"2000","journal-title":"Blood"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2014.03.007"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000107"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2017.08.015"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-020-04174-4"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-200310000-00007"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.1.105-114.2003"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2384"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64697-4"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.68.9.6069-6073.1994"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/173.3.529"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-020-0575-3"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1711750303"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2016.1169406"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAI.0000000000000562"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080131"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.4.1492"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12061479"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-70-7-1755"},{"key":"e_1_2_9_34_1","first-page":"35","article-title":"EBNA2 and its coactivator EBNA\u2010LP","volume":"391","author":"Kempkes B","year":"2015","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-11-814913"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.14652","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/his.14652","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.14652","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T06:49:00Z","timestamp":1692859740000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.14652"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,4]]},"references-count":34,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,6]]}},"alternative-id":["10.1111\/his.14652"],"URL":"https:\/\/doi.org\/10.1111\/his.14652","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,4]]},"assertion":[{"value":"2021-12-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}