{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T00:51:35Z","timestamp":1787014295041,"version":"build-2736575974"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T00:00:00Z","timestamp":1651622400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001711","name":"Schweizerischer Nationalfonds zur F\u00f6rderung der Wissenschaftlichen Forschung","doi-asserted-by":"publisher","award":["S\u201087701\u201003\u201001"],"award-info":[{"award-number":["S\u201087701\u201003\u201001"]}],"id":[{"id":"10.13039\/501100001711","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      Eosinophilic solid and cystic renal cell carcinoma (ESC RCC) is a recently described renal tumour entity with frequent cytokeratin (CK)20 positivity, commonly harbouring\n                      <jats:italic>TSC<\/jats:italic>\n                      mutations. In contrast, frequency of CK20 expression and presence of\n                      <jats:italic>TSC<\/jats:italic>\n                      mutations are unclear in\n                      <jats:italic>TFEB<\/jats:italic>\n                      \u2010amplified RCC and\n                      <jats:italic>TFEB<\/jats:italic>\n                      \u2010translocated RCC, which frequently express Melan A. Herein, we provide a comparative analysis of six ESC RCC with four\n                      <jats:italic>TFEB<\/jats:italic>\n                      \u2010amplified\/translocated RCC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and results<\/jats:title>\n                    <jats:p>\n                      We assessed the frequency of CK20 and Melan A expression by immunohistochemistry and of\n                      <jats:italic>TSC<\/jats:italic>\n                      mutations by next\u2010generation sequencing.\n                      <jats:italic>TFEB<\/jats:italic>\n                      alterations were confirmed by fluorescence\n                      <jats:italic>in\u2010situ<\/jats:italic>\n                      hybridisation (FISH). All tumours showed voluminous eosinophilic cells with granular cytoplasm, prominent nucleoli, and most showed admixture of solid and cystic areas. CK20 expression was found in all six ESC RCC and in all RCCs with\n                      <jats:italic>TFEB<\/jats:italic>\n                      alterations. Melan A positivity was identified in five of six ESC RCC and four of four RCC with\n                      <jats:italic>TFEB<\/jats:italic>\n                      alterations. We found\n                      <jats:italic>TSC<\/jats:italic>\n                      mutations in two ESC RCCs, including in one case also harbouring a\n                      <jats:italic>CIC<\/jats:italic>\n                      fusion, and identified a\n                      <jats:italic>TSC<\/jats:italic>\n                      mutation in one\n                      <jats:italic>TFEB<\/jats:italic>\n                      \u2010amplified RCC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>ESC RCC represents an emerging renal tumour entity with some histological, immunohistochemical and molecular overlap to TFEB\u2010amplified\/translocated RCC. FISH for TFEB aids in this differential diagnosis in challenging cases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.14663","type":"journal-article","created":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T07:42:44Z","timestamp":1649662964000},"page":"32-43","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Eosinophilic solid and cystic renal cell carcinoma and renal cell carcinomas with\n                    <scp>\n                      <i>TFEB<\/i>\n                    <\/scp>\n                    alterations: a comparative study"],"prefix":"10.1111","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6829-1391","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Lobo","sequence":"first","affiliation":[{"name":"Department of Pathology, Portuguese Oncology Institute of Porto (IPOP) R. Dr Ant\u00f3nio Bernardino de Almeida  Porto Portugal"},{"name":"Cancer Biology and Epigenetics Group, Research Center of IPO Porto (GEBC CI\u2010IPOP) \/ RISE@CI\u2010IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto) and Porto Comprehensive Cancer Center, (P.CCC) R. Dr. Ant\u00f3nio Bernardino de Almeida  Porto Portugal"},{"name":"Department of Pathology and Molecular Immunology, ICBAS\u2013School of Medicine and Biomedical Sciences University of Porto (ICBAS\u2010UP)  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Rechsteiner","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit M","family":"Helmchen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7043-3456","authenticated-orcid":false,"given":"Niels J","family":"Rupp","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University Hospital Zurich  Zurich Switzerland"},{"name":"Faculty of Medicine University of Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achim","family":"Weber","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University Hospital Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Moch","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University Hospital Zurich  Zurich Switzerland"},{"name":"Faculty of Medicine University of Zurich  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2015.02.003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000203"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000720"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.21037\/tau-20-1150"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11081110"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3182613d8f"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001067"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2018.06.007"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000838"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001111"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-021-00816-8"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.26502\/anu.2644-2833019"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000508"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2018.05.023"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2018.06.040"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000248"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000237"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.13395"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.13457"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/1066896921993229"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/10668969211038737"},{"key":"e_1_2_8_23_1","first-page":"1418","article-title":"The 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