{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T13:35:24Z","timestamp":1792157724449,"version":"4.4.0"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,5,5]],"date-time":"2022-05-05T00:00:00Z","timestamp":1651708800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["PTDC\/MEC\u2010URO\/ 29043\/2017"],"award-info":[{"award-number":["PTDC\/MEC\u2010URO\/ 29043\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["SFRH\/BD\/132751\/2017"],"award-info":[{"award-number":["SFRH\/BD\/132751\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      Somatic malignant transformation (SMT) arising in germ cell tumours (GCTs) is an infrequent, but clinically relevant event. There is only limited knowledge on the morphological spectrum of SMT, and the therapeutic management of these patients is poorly defined. In this work we revisit two consecutive case series (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u2009756) of GCTs. Clinicopathological data of SMTs arising in GCTs were determined, with a focus on the histopathological spectrum, and molecular aspects were obtained by Fluorescence\n                      <jats:italic>in\u2009situ<\/jats:italic>\n                      Hybridization (FISH) and Next Generation Sequencing (NGS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods and Results<\/jats:title>\n                    <jats:p>\n                      Thirty male patients (28 primary testicular, two primary extragonadal) were included. These patients represent 4% of GCT patients diagnosed at two institutes (University Hospital Zurich and IPO Porto). The most common SMTs were adenocarcinoma (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098), embryonic\u2010type neuroectodermal tumours (ENETs,\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098), and rhabdomyosarcoma (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20096), but a wide range of challenging morphologies were depicted, including low\u2010grade neuroglial tumour, adenosquamous carcinoma, neuroblastoma, and neuroendocrine carcinoma. SMT was found in 15 primary tumour samples and in 27 metastatic samples of these 30 patients, the latter showing poorer overall survival. Adenocarcinoma occurred only in metastasis postchemotherapy and in one primary retroperitoneal GCT with SMT, but not in GCT of the testis. The 12p gains were identified by FISH in all cases. NGS results were available in six patients. Clinical trials and\/or targeted treatments based on the molecular profile of SMT were recommended in four patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>SMT arising in GCTs represent a diagnostic challenge and should be confirmed by a specialized uropathologist. NGS\u2010based treatment recommendations may improve the outcome of these patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/his.14667","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T05:14:11Z","timestamp":1650345251000},"page":"84-98","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Morphological spectrum and molecular features of somatic malignant transformation in germ cell tumours"],"prefix":"10.1111","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6829-1391","authenticated-orcid":false,"given":"Jo\u00e3o","family":"Lobo","sequence":"first","affiliation":[{"name":"Department of Pathology Portuguese Oncology Institute of Porto (IPOP), R. Dr. Ant\u00f3nio Bernardino de Almeida  Porto Portugal"},{"name":"Cancer Biology and Epigenetics Group IPO Porto Research Center (GEBC CI\u2010IPOP) \/ RISE@CI\u2010IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto) \/ Porto Comprehensive Cancer Center (P.CCC), R. Dr. Ant\u00f3nio Bernardino de Almeida  Porto Portugal"},{"name":"Department of Pathology and Molecular Immunology ICBAS\u2013School of Medicine and Biomedical Sciences, University of Porto (ICBAS\u2010UP), Rua Jorge Viterbo Ferreira 228  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c2ngelo","family":"Rodrigues","sequence":"additional","affiliation":[{"name":"Department of Pathology Portuguese Oncology Institute of Porto (IPOP), R. Dr. Ant\u00f3nio Bernardino de Almeida  Porto Portugal"},{"name":"Cancer Biology and Epigenetics Group IPO Porto Research Center (GEBC CI\u2010IPOP) \/ RISE@CI\u2010IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto) \/ Porto Comprehensive Cancer Center (P.CCC), R. Dr. Ant\u00f3nio Bernardino de Almeida  Porto Portugal"},{"name":"Department of Pathology and Molecular Immunology ICBAS\u2013School of Medicine and Biomedical Sciences, University of Porto (ICBAS\u2010UP), Rua Jorge Viterbo Ferreira 228  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Henrique","sequence":"additional","affiliation":[{"name":"Department of Pathology Portuguese Oncology Institute of Porto (IPOP), R. Dr. Ant\u00f3nio Bernardino de Almeida  Porto Portugal"},{"name":"Cancer Biology and Epigenetics Group IPO Porto Research Center (GEBC CI\u2010IPOP) \/ RISE@CI\u2010IPOP (Health Research Network), Portuguese Oncology Institute of Porto (IPO Porto) \/ Porto Comprehensive Cancer Center (P.CCC), R. Dr. Ant\u00f3nio Bernardino de Almeida  Porto Portugal"},{"name":"Department of Pathology and Molecular Immunology ICBAS\u2013School of Medicine and Biomedical Sciences, University of Porto (ICBAS\u2010UP), Rua Jorge Viterbo Ferreira 228  Porto Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ailsa","family":"Christiansen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University Hospital Zurich, Schmelzbergstrasse 12  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Beyer","sequence":"additional","affiliation":[{"name":"Universit\u00e4tsklinik f\u00fcr Medizinische Onkologie Inselspital, Universit\u00e4tsklinik der Universit\u00e4t Bern, Bern University  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Moch","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University Hospital Zurich, Schmelzbergstrasse 12  Zurich Switzerland"},{"name":"Faculty of Medicine University of Zurich, R\u00e4mistrasse 71  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter Karl","family":"Bode","sequence":"additional","affiliation":[{"name":"Department of Pathology and Molecular Pathology University Hospital Zurich, Schmelzbergstrasse 12  Zurich Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,5]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"Global cancer observatory: cancer today","author":"Ferlay J","year":"2020"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2018.07.016"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2019.07.01"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20020258"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2021.07.037"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12061601"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737140.2018.1450630"},{"key":"e_1_2_10_9_1","volume-title":"Who classification of tumours of the urinary system and male genital organs (4th edition)","author":"Moch H","year":"2016"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0003134820919738"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mph.0000212992.72059.eb"},{"key":"e_1_2_10_12_1","first-page":"5273607","article-title":"Somatic malignant transformation of a testicular teratoma: a case report and an unusual presentation","volume":"2019","author":"Ibrahim DY","year":"2019","journal-title":"Case. Rep. Pathol."},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergencyto.2008.02.012"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOU.0000000000000528"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.21037\/tau.2019.09.08"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2490-13-9"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001352"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e3181adb9d7"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12123755"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19850815)56:4<860::AID-CNCR2820560426>3.0.CO;2-3"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001789"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2010.09705.x"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1177\/1066896910390680"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.21873\/anticanres.13678"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-012-1251-x"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2014.05.118"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2015.12.082"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318033c7c4"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)63754-6"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)64035-7"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anndiagpath.2021.151735"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdq045"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2010.70"},{"key":"e_1_2_10_34_1","first-page":"1299","article-title":"\u201cEmbryonic\u2010type neuroectodermal tumor\u201d should replace \u201cprimitive neuroectodermal tumor\u201d of the testis and gynecologic tract: a rationale for new nomenclature","volume":"45","author":"Flood TA","year":"2021","journal-title":"LWW"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001206"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000001248"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19841101)54:9<1824::AID-CNCR2820540910>3.0.CO;2-J"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)66339-2"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1097\/COC.0b013e31829d1ed7"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0000000000000262"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1703212"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1097\/MPH.0000000000000823"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2003.01.019"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2006.10.021"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.22397"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-018-0476-y"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000478-199505000-00005"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000003378"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.5152\/ttd.2014.4280"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00383-007-2006-7"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-942272"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.14667","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/his.14667","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.14667","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T03:15:05Z","timestamp":1692933305000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.14667"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,5]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1111\/his.14667"],"URL":"https:\/\/doi.org\/10.1111\/his.14667","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,5]]},"assertion":[{"value":"2022-01-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}