{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T11:39:08Z","timestamp":1774611548462,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T00:00:00Z","timestamp":1660262400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100011297","name":"United States and Canadian Academy of Pathology","doi-asserted-by":"publisher","award":["NIHP50HD098580"],"award-info":[{"award-number":["NIHP50HD098580"]}],"id":[{"id":"10.13039\/100011297","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007059","name":"Northwestern University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007059","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:p>HMGA2 overexpression is found in 10\u201315% of leiomyomas (LM). HMGA2 overexpression is common in variants of hydropic, intravenous and lipo\u2010LM. Cellular or highly cellular LM (CLM) is a LM variant with a less well\u2010defined molecular nature. In this study, we identified and examined 52 hypercellular LM with sclerotic collagen, herein defined as cellular leiomyoma with sclerosis (CLM\u2010S). CLM\u2010S shows large tumour size (average 12.2\u2009cm) and characteristic histology of tumour cells, arranged in cellular fascicles, sheets and trabeculae with abundant dense, pink sclerotic extracellular matrix in bands and nodules and increased vascularity. Tumour cells are uniform with small, round\u2013oval nuclei and scant, pale\u2013eosinophilic to vacuolated cytoplasm reminiscent of pericytes. The differential diagnosis of CLM\u2010S includes conventional CLM, endometrial stromal tumours and perivascular epithelioid cell tumour. Immunohistochemical profile [HMGA2, fumarate hydratase, smooth muscle markers, Melan A and HMB\u201045] and molecular alterations [by HMGA2 mRNA reverse transcription\u2013polymerase chain reaction (RT\u2013PCR), <jats:italic>HMGA2<\/jats:italic> fluorescence <jats:italic>in\u2010situ<\/jats:italic> hybridisation and <jats:italic>MED12<\/jats:italic> sequencing] were analysed in comparison to matched myometrium and CLM controls. Remarkably, 96% (50 of 52) of CLM\u2010S demonstrated diffuse positive immunoreactivity for HMGA2 and up to an 80\u2010fold increase in HMGA2 mRNA, determined by RT\u2013PCR. FISH analysis with break\u2010part probes at intron 3 and the 5' UTR detected <jats:italic>HMGA2<\/jats:italic> rearrangements in 47% (18 of 38) of CLM\u2010S. All CLM\u2010S retained expression of fumarate hydratase. No <jats:italic>MED12<\/jats:italic> mutations were found in any CLM\u2010S. Our findings show that CLM\u2010S has unique and characteristic histomorphology probably driven by HMGA2 overexpression.<\/jats:p>","DOI":"10.1111\/his.14732","type":"journal-article","created":{"date-parts":[[2022,7,30]],"date-time":"2022-07-30T08:46:14Z","timestamp":1659170774000},"page":"587-599","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Histological and molecular analysis of cellular leiomyoma with sclerosis: linked to <scp>HMGA2<\/scp> overexpression"],"prefix":"10.1111","volume":"81","author":[{"given":"Brannan B","family":"Griffin","sequence":"first","affiliation":[{"name":"Department of Pathology Northwestern University Feinberg School of Medicine  Chicago IL USA"},{"name":"Department of Pathology, Microbiology and Immunology Vanderbilt University Medical Center  Nashville TN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Pathology Northwestern University Feinberg School of Medicine  Chicago IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priyanka","family":"Saini","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Northwestern University Feinberg School of Medicine  Chicago IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyan","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pathology Northwestern University Feinberg School of Medicine  Chicago IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Serdar","family":"Bulun","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Northwestern University Feinberg School of Medicine  Chicago IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Debabrata","family":"Chakravarti","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Northwestern University Feinberg School of Medicine  Chicago IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0281-2793","authenticated-orcid":false,"given":"Jian\u2010Jun","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Pathology Northwestern University Feinberg School of Medicine  Chicago IL USA"},{"name":"Department of Obstetrics and Gynecology Northwestern University Feinberg School of Medicine  Chicago IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,12]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"272","volume-title":"Uterine Leiomyoma","author":"Ip C","year":"2020"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1209993"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1302736"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.215"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-17-1744"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2018.09.012"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2016.36"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-4608(02)00568-X"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/PGP.0000000000000141"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2017.12.005"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.path.2019.01.004"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-021-03217-z"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0050"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.243"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22888"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-017-0672-1"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.28900"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41379-021-00946-z"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2020.00538"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1540407"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1137999"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20415"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/5.12.1150"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.14732","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/his.14732","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.14732","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T22:55:34Z","timestamp":1692658534000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.14732"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,12]]},"references-count":23,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1111\/his.14732"],"URL":"https:\/\/doi.org\/10.1111\/his.14732","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,12]]},"assertion":[{"value":"2022-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-12","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}