{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T09:06:15Z","timestamp":1790845575641,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,10,18]],"date-time":"2022-10-18T00:00:00Z","timestamp":1666051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>Low\u2010grade oncocytic tumour (LOT) of the kidney has recently emerged as a potential novel tumour type. Despite similarity to oncocytoma or eosinophilic chromophobe renal cell carcinoma, it shows diffuse keratin 7 immunohistochemistry (IHC) and negative KIT (CD117), which differs from both. We aimed to identify the molecular characteristics of these tumours. Seventeen tumours (one male, 16 female, nine previously published) fitting the original description of this entity (solid eosinophilic cell morphology, often with areas of tumour cells loosely stretched in oedematous stroma, and the above IHC features) were analysed with a next\u2010generation sequencing panel of 324 cancer\u2010associated genes from formalin\u2010fixed, paraffin\u2010embedded tissue. All tumours harboured at least one alteration in either <jats:italic>TSC1<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u00a07, 41%), <jats:italic>TSC2<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u00a02, 12%), <jats:italic>MTOR<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u00a05, 29%) or <jats:italic>PIK3CA<\/jats:italic> (<jats:italic>n<\/jats:italic>\u00a0=\u00a04, 24%). Four tumours harboured a second alteration, including two <jats:italic>NF2<\/jats:italic>, one each in conjunction with <jats:italic>MTOR<\/jats:italic> and\u00a0<jats:italic>TSC2<\/jats:italic> alterations, one <jats:italic>PTEN<\/jats:italic> with <jats:italic>TSC1<\/jats:italic> alteration and one tumour with both <jats:italic>MTOR<\/jats:italic> and <jats:italic>TSC1<\/jats:italic> alterations. No other renal cancer\u2010related or recurring gene alterations were identified. In addition to the previously described IHC findings, 16 of 16 were positive for GATA3. Eleven patients with follow\u2010up had no metastases or recurrent tumours. Recurrent tuberous sclerosis\/MTOR pathway gene alterations in LOT support its consideration as a distinct morphological, immunohistochemical and genetic entity. <jats:italic>PIK3CA<\/jats:italic> is another pathway member that may be altered in these tumours. Further study will be necessary to determine whether tumour behaviour or syndromic associations differ from those of oncocytoma and chromophobe carcinoma, warranting different clinical consideration.<\/jats:p>","DOI":"10.1111\/his.14816","type":"journal-article","created":{"date-parts":[[2022,10,8]],"date-time":"2022-10-08T08:00:04Z","timestamp":1665216004000},"page":"296-304","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["Low\u2010grade oncocytic tumour of the kidney is characterised by genetic alterations of <i>TSC1<\/i>, <i>TSC2<\/i>, <i>MTOR<\/i> or <i>PIK3CA<\/i> and consistent <scp>GATA3<\/scp> positivity"],"prefix":"10.1111","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3898-1460","authenticated-orcid":false,"given":"Sean R","family":"Williamson","sequence":"first","affiliation":[{"name":"Department of Pathology Robert J. Tomsich Pathology and Laboratory Medicine Institute, Cleveland Clinic  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3236-3127","authenticated-orcid":false,"given":"Ondrej","family":"Hes","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine in Pilsen Charles University  Pilsen Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3142-8846","authenticated-orcid":false,"given":"Kiril","family":"Trpkov","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Alberta Precision Labs and University of Calgary  Calgary Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditi","family":"Aggarwal","sequence":"additional","affiliation":[{"name":"CORE Diagnostics  Gurgaon Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abhishek","family":"Satapathy","sequence":"additional","affiliation":[{"name":"Advanced Medical Research Institute  Bhubaneswar Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sourav","family":"Mishra","sequence":"additional","affiliation":[{"name":"Advanced Medical Research Institute  Bhubaneswar Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivani","family":"Sharma","sequence":"additional","affiliation":[{"name":"CORE Diagnostics  Gurgaon Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0874-8964","authenticated-orcid":false,"given":"Ankur","family":"Sangoi","sequence":"additional","affiliation":[{"name":"Department of Pathology El Camino Hospital  Mountain View CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6049-5293","authenticated-orcid":false,"given":"Liang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University School of Medicine  Indianapolis IN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmut","family":"Akgul","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Albany Medical Center  Albany NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6611-6389","authenticated-orcid":false,"given":"Muhammad","family":"Idrees","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Indiana University School of Medicine  Indianapolis IN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Levin","sequence":"additional","affiliation":[{"name":"Department of Public Health Sciences Henry Ford Hospital  Detroit MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sudha","family":"Sadasivan","sequence":"additional","affiliation":[{"name":"Department of Public Health Sciences Henry Ford Hospital  Detroit MI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"San Miguel Fraile","sequence":"additional","affiliation":[{"name":"Hospital \u00c1lvaro Cunqueiro, Vigo  Pontevedra Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Rogala","sequence":"additional","affiliation":[{"name":"Regional Specialist Hospital  Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8488-543X","authenticated-orcid":false,"given":"Eva","family":"Comperat","sequence":"additional","affiliation":[{"name":"Department of Pathology, H\u00f4pital Tenon Sorbonne University, Paris VI  Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5474-8696","authenticated-orcid":false,"given":"Daniel M","family":"Berney","sequence":"additional","affiliation":[{"name":"Department of Cellular Pathology, Bartshealth NHS Trust and Barts Cancer Institute Queen Mary University of London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stela","family":"Bulimbasic","sequence":"additional","affiliation":[{"name":"Department of Pathology School of Medicine  Zagreb Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0851-1465","authenticated-orcid":false,"given":"Jesse K","family":"McKenney","sequence":"additional","affiliation":[{"name":"Department of Pathology Robert J. Tomsich Pathology and Laboratory Medicine Institute, Cleveland Clinic  Cleveland Ohio USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shilpy","family":"Jha","sequence":"additional","affiliation":[{"name":"Advanced Medical Research Institute  Bhubaneswar Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nakul Y","family":"Sampat","sequence":"additional","affiliation":[{"name":"Advanced Medical Research Institute  Bhubaneswar Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sambit K","family":"Mohanty","sequence":"additional","affiliation":[{"name":"CORE Diagnostics  Gurgaon Haryana India"},{"name":"Advanced Medical Research Institute  Bhubaneswar Odisha India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,10,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.13865"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12010168"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-020-02927-0"},{"key":"e_1_2_7_5_1","first-page":"6684777","article-title":"A case of low\u2010grade oncocytic tumor\/chromophobe renal cell carcinoma (oncocytic variant) of the kidney","volume":"2021","author":"Ishikawa N","year":"2021","journal-title":"Case Rep. 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