{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T04:33:50Z","timestamp":1789792430475,"version":"4.0.1"},"reference-count":52,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,11,9]],"date-time":"2022-11-09T00:00:00Z","timestamp":1667952000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:p>Lymph node micrometastases could be one of the reasons for the high recurrence rate after complete surgical resection in stage I\u2013IIIA non\u2010small cell lung cancer (NSCLC). The standard evaluation of a single haematoxylin and eosin (H&amp;E) slide of a paraffin\u2010embedded section of a lymph node is insufficient for the detection of micrometastases, and there is a need for additional\u00a0histopathological evaluation. The association of lymph\u00a0node micrometastases with survival remains as\u00a0yet unresolved. The aim of this systematic review and meta\u2010analysis is to investigate if lymph node micrometastases and isolated tumour cells in patients with stage I\u2013IIIA NSCLC, detected with multiple sectioning and\/or immunohistochemistry (IHC) and\/or reverse transcriptase polymerase chain reaction (RT\u2010PCR), are associated with overall survival (OS) and disease\u2010free survival (DFS) after surgical resection. We performed a meta\u2010analysis of time\u2010to\u2010event outcomes based on 15 articles using ancillary techniques to detect micrometastases. We extracted the OS and DFS every 3\u20136\u2009months after surgery, for patients with and without occult lymph node micrometastasis, from the survival curves published in each article. These data were used to reconstruct OS and DFS for \u2018micrometastasis\u2019 and \u2018no micrometastasis\u2019 groups. Based on all included studies that used IHC, serial sectioning, or RT\u2010PCR, we found a 5\u2010year OS of 55% (micrometastasis) vs. 75% (no micrometastasis), and a 5\u2010year DFS of 53% (micrometastasis) vs. 75% (no micrometastasis). Patients with stage I\u2013IIIA NSCLC with lymph node micrometastases detected by ancillary histopathological and molecular techniques have a significantly poorer OS and DFS compared to patients without lymph node micrometastases.<\/jats:p>","DOI":"10.1111\/his.14831","type":"journal-article","created":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T09:04:26Z","timestamp":1666688666000},"page":"650-663","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Systematic review and meta\u2010analysis of the prognostic impact of lymph node micrometastasis and isolated tumour cells in patients with stage\n                    <scp>I\u2013IIIA<\/scp>\n                    non\u2010small cell lung cancer"],"prefix":"10.1111","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2603-0115","authenticated-orcid":false,"given":"Melek","family":"H\u00fcy\u00fck","sequence":"first","affiliation":[{"name":"Department of 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